We narrowed to 59,421 results for: cloning

1. pDONR223 GABARAPL1 G116A

   Plasmid

   #123084

   Purpose

   Gateway entry clone encoding human GABARAPL1 G116A

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Glycine 116 to Alanine

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HE401: pMVP (L1-R5) shRNA

   Plasmid

   #121698

   Purpose

   pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-kcnj11

   Plasmid

   #173139

   Purpose

   This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

   Insert

   kcnj11 (kcnj11 Zebrafish)
   

   Use

   Pcr cloning vector

   Promoter

   No promoter at 5 end; T7 promoter at 3 end.

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. JZ901: pMVP (L5-L4) EF1a promoter

   Plasmid

   #121727

   Purpose

   pMVP L5-L4 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   EF1a promoter
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. IG001: pMVP (L1-L4) TBG promoter

   Plasmid

   #121689

   Purpose

   pMVP L1-L4 entry plasmid, contains TBG promoter for 3-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   TBG promoter (Liver specific)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. JT504: pMVP/SB/eGFP-DEST

   Plasmid

   #121861

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. JT601: pMVP/SB/mCherry-DEST

   Plasmid

   #121862

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. LF601: pMVP (L1-R5) TBG promoter

   Plasmid

   #121695

   Purpose

   pMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   TBG promoter (Liver specific)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-mouse TCR zeta/2451

   Plasmid

   #11506

   Depositor

   Allan Weissman

   Article

   Weissman et al Science. 1988 Feb 26. 239(4843):1018-21.

   Insert

   TCR zeta (Cd247 Mouse)
   

   Use

   General cloning

   Mutation

   n/a

   Available Since

   March 8, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid

    #140200

    Purpose

    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    dLbCas12a crRNA scaffold

    Promoter

    PgpdA

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-FRB(PLF)-HA no-pA

    Plasmid

    #82597

    Purpose

    3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRB-HA (no pA)
    

    Mutation

    K2095P, T2098L, W2101F

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223 GABARAP G116

    Plasmid

    #123082

    Purpose

    Gateway entry clone encoding human GABARAP G116

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. KN302: pMVP/SB/eCFP-DEST

    Plasmid

    #121863

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KK901: pMVP/SB/Zeo-DEST

    Plasmid

    #121868

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. KJ101: pMVP (R4-R3) osTir1

    Plasmid

    #121742

    Purpose

    pMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    osTir1
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    9x myc epitope tag

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. JN402: pMVP (R4-R3) Firefly Luciferase

    Plasmid

    #121741

    Purpose

    pMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Firefly Luciferase
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. IG603: pMVP (L1-L4) TRE promoter

    Plasmid

    #121691

    Purpose

    pMVP L1-L4 entry plasmid, contains TRE promoter for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOD_C3106

    Plasmid

    #91101

    Purpose

    Module C, Promoter: FMV 34S, Gene: TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Promoter

    FMV 34S

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVC-Ds-DrU6a:sgRNA-Ds

    Plasmid

    #119069

    Purpose

    To clone sgRNA spacer sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:sgRNA
    

    Expression

    Bacterial

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOD_C4300

    Plasmid

    #91103

    Purpose

    Module C, Promoter: none, Gene: BaeI LacZ cassette for GT donor cloning, Terminator: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BaeI LacZ cassette for GT donor cloning
    

    Use

    Unspecified

    Promoter

    none

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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