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pEPCTΩSP0021

Plasmid

#187561

Purpose

Module for copper-inducible expression of firefly luciferase and nanoluc luciferase driven by minimal synthetic promoters with binding sites for CUP2

Depositor

Nicola Patron

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Firefly Luciferase

Expression

Plant

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEPCTΩSP0022

Plasmid

#187562

Purpose

Module for copper-inducible expression of nanoluc luciferase and firefly luciferase driven by minimal synthetic promoters with binding sites for CUP2

Depositor

Nicola Patron

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Firefly Luciferase and NanoLuciferase

Expression

Plant

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPIBY55

Plasmid

#213706

Purpose

Express the affinity sorting tag TST-EGFP- GPIBY55.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPICEAM7

Plasmid

#213705

Purpose

Express the affinity sorting tag TST-EGFP- GPICEAM7.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTST-EGFP-GPIDAF

Plasmid

#213704

Purpose

Express the affinity sorting tag TST-EGFP- GPIDAF.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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β2AR-PPI-CALPon1.0 (TEVp)

Plasmid

#213407

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

HA-arrestin-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSECB sgCASP9

Plasmid

#211525

Purpose

Deletes CASP9

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgCASP9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgFADD

Plasmid

#211527

Purpose

Deletes FADD

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgPIDD

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUCKL1

Plasmid

#211523

Purpose

Deletes UCKL1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCKL1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUCK2

Plasmid

#211522

Purpose

Deletes UCK2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCK2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgUGP2

Plasmid

#211521

Purpose

Deletes UGP2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUGP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.2

Plasmid

#208303

Purpose

Expresses ABE-RA3.2 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.2-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, P48A, R51H, D108G, K110R, A114V, H122R, M12…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.3

Plasmid

#208304

Purpose

Expresses ABE-RA3.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.3-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, A114V…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.0

Plasmid

#208305

Purpose

Expresses ABE-RA4.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.0-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-yATOM_SH2-KDEL

Plasmid

#209714

Purpose

Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-yATOM_SH2-KDEL

Tags

PRLSS

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_WDR5 (R80A)

Plasmid

#209713

Purpose

Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_WDR5 (R80A)

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

Plasmid

#209718

Purpose

Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-NbGFP_miRFP670nano3-KDEL

Tags

PRLSS

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-Clover-KDEL

Plasmid

#209720

Purpose

Mammalian expression of clover localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-Clover-KDEL

Tags

PRLSS

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-yATOM_SH2

Plasmid

#209715

Purpose

Mammalian expression of y-ATOM biosensor for SH2 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-yATOM_SH2

Tags

Cos4

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Bamh1

Plasmid

#203303

Purpose

For expression of eukaryotic genes of interest with CMV promoter with Bamh1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Nhe1

Plasmid

#203304

Purpose

For expression of eukaryotic genes of interest with CMV promoter with NheI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PlayBack-CMV-Bgl11

Plasmid

#203306

Purpose

For expression of eukaryotic genes of interest with CMV promoter with BglII as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PlayBack-Ef1a-Nde1

Plasmid

#203309

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with NdeI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PlayBack-Ef1a-Nhe1

Plasmid

#203310

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with NheI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXSIP-TMEM141-FLAG

Plasmid

#204702

Purpose

expresses TMEM141-FLAG in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

TMEM141 (TMEM141 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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GB3682

Plasmid

#187807

Purpose

Transcriptional unit for expression of alcohol O-acetyltransferase from Saccharomyces pastorianus strain CBS 1483 chromosome SeVIII-SeXV, codon optimized for Nicotiana.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

SpATF1-2

Expression

Plant

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBZS-YP_009701874

Plasmid

#198378

Purpose

Express YP_009701874 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_009701874 (CVA-1_543R )

Expression

Bacterial

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBZS-YP_001426001

Plasmid

#198361

Purpose

Express pBZS-YP_001426001 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_001426001 (N369L )

Expression

Bacterial

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBZS-YP_009665506

Plasmid

#198376

Purpose

Express YP_009665506 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_009665506 (NYs-1_727R )

Expression

Bacterial

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBZS-YP_009665369

Plasmid

#198375

Purpose

Express YP_009665369 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_009665369 (NYs-1_421L )

Expression

Bacterial

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge