We narrowed to 15,189 results for: otos

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pTAG4E

Plasmid

#176868

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG5

Plasmid

#176869

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_5

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG6E

Plasmid

#176870

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_6E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-CmYLCV-6

Plasmid

#176872

Purpose

MoClo Level 0 plasmid, contains CmYLCV-6 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A4800EC

Plasmid

#176873

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_B4800EC

Plasmid

#176874

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2

Plasmid

#176864

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG3

Plasmid

#176866

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_3

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG4

Plasmid

#176867

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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CSM160

Plasmid

#105684

Purpose

Plasmid containing an RFP dropout used to construct randomized PAM library

Depositor

Chase Beisel

Article

Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

Insert

RFP Golden Gate dropout

Expression

Bacterial

Promoter

Bba_R0040 TetR Promoter

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3137

Plasmid

#179617

Purpose

Punc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

GCaMP6

Tags

RFP

Expression

Worm

Promoter

Punc-4

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2157

Plasmid

#179204

Purpose

Pacr-2::Ca -insensitive D3cpv unc-54 3' UTR C.elegans neural expression of Pacr-2 calcium D3cpv

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

D3cpv

Expression

Worm

Promoter

Pacr-2

Available Since

Feb. 22, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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AP-VAMP1(X)

Plasmid

#167999

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP1(L56-L72)-T7PAP

Use

Synthetic Biology

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#2

Plasmid

#163395

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh2 gRNA#2

Plasmid

#163398

Purpose

Cas9-mediated knockout of Ssh2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh2 (Ssh2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh2 gRNA#1

Plasmid

#163397

Purpose

Cas9-mediated knockout of Ssh2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh2 (Ssh2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#1

Plasmid

#163394

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#3

Plasmid

#163396

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh2 gRNA#3

Plasmid

#163399

Purpose

Cas9-mediated knockout of Ssh2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh2 (Ssh2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh3 gRNA#2

Plasmid

#163401

Purpose

Cas9-mediated knockout of Ssh3 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh3 (Ssh3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh3 gRNA#3

Plasmid

#163402

Purpose

Cas9-mediated knockout of Ssh3 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh3 (Ssh3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Rhoa

Plasmid

#163605

Purpose

Expresses RhoA Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Rhoa (Rhoa Mouse)

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh3 gRNA#1

Plasmid

#163400

Purpose

Cas9-mediated knockout of Ssh3 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh3 (Ssh3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-Dnase1l1l-mRFP

Plasmid

#169002

Purpose

Express zebrafish Dnase1l1l-mRFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Dnase1l1l-mRFP (dnase1l3l Zebrafish)

Use

Unspecified

Tags

mRFP

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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AP-VAMP5

Plasmid

#168001

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP5(L28-L44)-T7PAP

Use

Synthetic Biology

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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R2x2_VAL88

Plasmid

#160761

Purpose

Expresses R2x2_VAL88 in bacterial cells

Depositor

Nobuyasu Koga

Article

Koga et al Proc Natl Acad Sci U S A. 2020 Nov 23. p

Insert

R2x2_VAL88

Tags

6xHis

Expression

Bacterial

Mutation

10-residue mutations of Rsmn2x2_5_6

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - L39V)

Plasmid

#136607

Purpose

Expresses DANCER-3 mutant (reversion L39V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (L39V reversion)

Tags

5x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - DANCER-2

Plasmid

#136386

Purpose

Expresses DANCER-2 dynamic protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

DANCER-2

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - NERD-S

Plasmid

#136388

Purpose

Expresses NERD-S static protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

NERD-S

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39I-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - NERD-C

Plasmid

#136389

Purpose

Expresses NERD-C static protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

NERD-C

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-F30L-V39I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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