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pCMV-myc3-Mdm2 p60

Plasmid

#52059

Purpose

expression of truncated Mdm2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Mdm2 p60 (truncated) (MDM2 Human)

Tags

myc3

Expression

Mammalian

Mutation

p60 (truncated at D367, DVDP motif)

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_DST-1/1

Plasmid

#91288

Purpose

Protein expression and purification of human SH3 domain construct DST-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DST-1/1 (DST Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

Plasmid

#140203

Purpose

Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

crRNA array elcA

Use

CRISPR and Synthetic Biology

Promoter

PgpdA

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ:mTREK-1_cryst

Plasmid

#133269

Purpose

P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLCHKO_TK1_HPRT1_Lb_4

Plasmid

#155062

Purpose

Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF1CACTA1_3xFLAG_TET1cd

Plasmid

#106434

Purpose

pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

TET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)

Use

Synthetic Biology

Tags

3xFLAG

Expression

Plant

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJJR50

Plasmid

#75026

Purpose

U6 promoter driven flipped + extended sgRNA expression vector

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

guide RNA, flipped and extended version

Use

CRISPR

Promoter

R07E5.16 (U6)

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_TET1cd

Plasmid

#106432

Purpose

pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

pUBQ10::ZF108_3xFLAG_TET1cd (TET1 Mustard Weed, Synthetic, Human)

Use

Synthetic Biology

Expression

Plant

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHalo2X-mMYO7A-ΔSH3-ΔM/F2

Plasmid

#251427

Purpose

Mammalian expression vector for expressing HaloTag-fused mouse MYO7A truncated after the first MyTH4-FERM domain

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin VIIa (SH3 and M/F2 trancated) (Myo7a Mouse)

Tags

HaloTag

Expression

Mammalian

Mutation

The SH3 and M/F2 domains trancated

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-CaBLAM

Plasmid

#244129

Purpose

Bioluminescent calcium indicator

Depositor

Nathan Shaner

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Insert

CaBLAM

Expression

Mammalian

Mutation

Calmodulin N98I

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_RPP29 (pAVA3545)

Plasmid

#239314

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP29

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting RPP29 (POP4 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_POP5 (pAVA3498)

Plasmid

#239315

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting POP5

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting POP5 (POP5 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_RPP14 (pAVA3507)

Plasmid

#239317

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP14

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting RPP14 (RPP14 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_DDX59 (pAVA3791)

Plasmid

#239297

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting DDX59 (DDX59 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_DDX59 (pAVA3317)

Plasmid

#239299

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting DDX59 (DDX59 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_DDX59 (pAVA3794)

Plasmid

#239300

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DDX59

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting DDX59 (DDX59 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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sgYAP-R-3

Plasmid

#229447

Purpose

knockout of YAP1

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-R-3 (YAP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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sgMARK2-3-2

Plasmid

#229441

Purpose

knockout of MARK2 and MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK2-3-2 (MARK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8ΔChromo

Plasmid

#224708

Purpose

Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 chromodomain truncation (CBX8 Human)

Tags

HMBP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsRUNX

Plasmid

#205639

Purpose

reRNA guide targeting Homo sapiens RUNX

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsRUNX (RUNX1 Human)

Expression

Mammalian

Promoter

U6

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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