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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,427 results for: ef1

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Showing: 3241 - 3260 of 9427 results

  1. VanR - F165V

    Plasmid
    #158965
    Purpose
    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.
    Depositor
    Michael Jensen
    Article
    et al Biotechnology Notes, Volume 1, 2020, Pag
    Insert
    VanR - F165V
    Expression
    Yeast
    Mutation
    Wild type VanR from Caulobacter crescentus with m…
    Promoter
    TEF1
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158965-plasmid-map-sequence-id-319279

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  2. VanR - F165R

    Plasmid
    #158966
    Purpose
    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.
    Depositor
    Michael Jensen
    Article
    et al Biotechnology Notes, Volume 1, 2020, Pag
    Insert
    VanR - F165R
    Expression
    Yeast
    Mutation
    Wild type VanR from Caulobacter crescentus with m…
    Promoter
    TEF1
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158966-plasmid-map-sequence-id-319292

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  10. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a core promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219823-plasmid-map-sequence-id-441127

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  17. CROPseq-Puro-4xMS2

    Plasmid
    #212626
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212626-plasmid-map-sequence-id-420274

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  19. pJT2

    Plasmid
    #98306
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(
    Insert
    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    Expression
    Yeast
    Mutation
    ERG20 N127W
    Available Since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    98306-plasmid-map-sequence-id-181809

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Showing: 3241 - 3260 of 9427 results