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Showing: 3241 - 3260 of 8369 results

  6. GFP Cav2.2 HA D665A pcDNA3

    Plasmid
    #206113
    Purpose
    expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional
    Depositor
    Annette Dolphin
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    D665A
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206113-plasmid-map-sequence-id-415392

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  7. cacna2d1 R241A D491A HA pcDNA3

    Plasmid
    #206109
    Purpose
    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding
    Depositor
    Annette Dolphin
    Insert
    cacna2d1 (Cacna2d1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R241A D491A
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206109-plasmid-map-sequence-id-415382

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  10. pcDNA3-FRB-VMAc-CSarcin

    Plasmid
    #70223
    Purpose
    Expresses VMA intein with C-terminal portion of alpha-sarcin
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (C-terminal portion)
    Use
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Asp 28 to Cys, deleted amino acids 1-27
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pcDNA3.1(+)Gαq-124-NLuc

    Plasmid
    #207153
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAQ (GNAQ Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position F(124)E
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207153-plasmid-map-sequence-id-410285

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Showing: 3241 - 3260 of 8369 results