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pOTTC385 - pAAV CMV-IE IRES EGFP

Plasmid

#102936

Purpose

An AAV vector that expresses IRES EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Publication

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

CMV-IE

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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8522-M01-663

Plasmid

#225660

Purpose

Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

Depositor

Dominic Esposito

Publication

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

SORE6-mCMVp

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-BMP2K

Plasmid

#23423

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMP2K (BMP2K Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA6.2-YFP-CWT

Plasmid

#176689

Purpose

Fluorescent reporter for phagosomal escape of Staphylococcus aureus

Depositor

Martin Fraunholz

Publication

Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

Insert

cell wall-targeting domain of Lysostaphin

Tags

YFP

Expression

Mammalian

Mutation

deletion of protase domain of Lysostaphin

Promoter

CMV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAT28-proD/SaEGFP

Plasmid

#120321

Purpose

Bacterial eGFP labeling

Depositor

James Kirby

Publication

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

eGFP with Staphylococcus aureus codon optimization

Expression

Bacterial

Promoter

proD

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-SacB

Plasmid

#113635

Purpose

Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

Depositor

Miguel Valvano

Publication

Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

Insert

sacB

Expression

Bacterial

Mutation

Please see Depositor Comments

Promoter

sacB promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pX330-NEAT1pr_v2

Plasmid

#97082

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Publication

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v2

Use

CRISPR

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-PCK2

Plasmid

#23715

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PCK2 (PCK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CDK2

Plasmid

#23777

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK2 (CDK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211462

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

Depositor

Agnel Sfeir

Publication

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pC-BAR-YR

Plasmid

#61766

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

Depositor

Ken Haynes

Publication

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Bar gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

trpC promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAB076i3

Plasmid

#79198

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-TLK2

Plasmid

#23629

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TLK2 (TLK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-CLK1

Plasmid

#23817

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CLK1 (CLK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pBS-pIgR cDNA

Plasmid

#12109

Depositor

Pamela Bjorkman

Publication

Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

Insert

human polymeric immunoglobulin receptor (PIGR Human)

Expression

Bacterial

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pPL5618_pUG_MtxR

Plasmid

#60929

Purpose

PCR template vector for generating methotrexate resistance cassette.

Depositor

Robert Piper

Publication

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Dihydrofolate reductase (DHFR Human)

Use

Loxp flanked deletion cassette template

Mutation

L22F F31S

Promoter

TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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B23(9-122)

Plasmid

#23142

Depositor

SeWon Suh

Publication

Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

Insert

B23 core (NPM1 Human)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-8

Available since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDONR223-PRKD3

Plasmid

#23580

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKD3 (PRKD3 Human)

Use

Gateway Cloning

Mutation

Q546R

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKAB2

Plasmid

#23647

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAB2 (PRKAB2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-NEK6

Plasmid

#23781

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK6 (NEK6 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article