We narrowed to 27,857 results for: sta

1. PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

   Plasmid

   #204497

   Purpose

   Expresses rtTA and Neomycin resistance genes in mammalian cells

   Depositor

   Wataru Fujii

   Article

   Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

   Insert

   rtTA
   

   Expression

   Mammalian

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUB_smFLAG_KDM5B_MS2

   Plasmid

   #81084

   Purpose

   Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smFLAG_KDM5B_24xMS2
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDG02583

   Plasmid

   #104129

   Purpose

   Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   NEDD8-specific protease 1 (NEDP1)
   

   Tags

   14x Histidine tag, Maltose binding protein, and S…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUGW-d2GFP-ZEB1 3'UTR

   Plasmid

   #79601

   Purpose

   FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

   Depositor

   Jeffrey Rosen

   Article

   Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

   Insert

   d2GFP-Zeb1 3'UTR
   

   Use

   Lentiviral

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMP71-Flt3L

   Plasmid

   #174600

   Purpose

   Secretes the extracellular domain of murine Flt3L

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   secretes extracellular domain of mouse Flt3-L
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-tetO-myc T58A

   Plasmid

   #19763

   Depositor

   Konrad Hochedlinger

   Article

   Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

   Insert

   c-myc (MYC Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T58A

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pROSA26-PA

   Plasmid

   #21271

   Depositor

   Frank Costantini

   Article

   Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Available Since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-EF1a/b-actin

   Plasmid

   #82583

   Purpose

   5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
   

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRESCA-syntide

   Plasmid

   #132415

   Purpose

   FRET-based sensor for CaMKII activity

   Depositor

   Margaret Stratton

   Article

   Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

   Insert

   FRESCA-syntide
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB602-TP-mCherry-UnaG

    Plasmid

    #203483

    Purpose

    For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    Plastid transit peptide fused mCherry-UnaG
    

    Expression

    Bacterial and Plant

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human ICAM-1

    Plasmid

    #8632

    Depositor

    Timothy Springer

    Article

    Staunton et al Cell 1988 Mar 25;52(6):925-33.

    Insert

    ICAM-1 (ICAM1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN HAYFP-LOV24

    Plasmid

    #49570

    Purpose

    regulate protein stability with light

    Depositor

    Thomas Wandless

    Article

    Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

    Insert

    YFP-LOV24
    

    Use

    Retroviral

    Tags

    HA and YFP

    Mutation

    delete the last 3 aa from AsLOV2, with I532A muta…

    Promoter

    LTR

    Available Since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ALK5 wt

    Plasmid

    #80876

    Purpose

    mammalian expression of ALK5 WT

    Depositor

    Aristidis Moustakas

    Article

    Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

    Insert

    ALK5 (TGFBR1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF-BFP

    Plasmid

    #138272

    Purpose

    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    BFP H66 PAM
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H66, PAM motif

    Promoter

    Ef1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWPI-TBX2

    Plasmid

    #185526

    Purpose

    Constitutive overexpression of human TBX2

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    TBX2 (TBX2 Human)
    

    Use

    Lentiviral

    Promoter

    EF-1a

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKanB

    Plasmid

    #223523

    Purpose

    Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

    Depositor

    Geoff Lin-Cereghino

    Article

    Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Sept. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. IRE1-2EGFP-ter-kanMX4

    Plasmid

    #184766

    Purpose

    Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    IRE1 (IRE1 Budding Yeast)
    

    Tags

    2EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HIR WT

    Plasmid

    #24049

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDT-sgRNA

    Plasmid

    #138271

    Purpose

    Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    sgBG, sgTS
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 7a sgRNA for EJ7-GFP and 4-μHOM reporters

    Plasmid

    #113620

    Purpose

    sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7a sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only