We narrowed to 2,346 results for: puc19

1. 3xFlag-HaloTag-SUZ12 HDR - pDY082

   Plasmid

   #171110

   Purpose

   Homology directed repair plasmid for generating HaloTag-SUZ12 cells

   Depositor

   Thomas Cech

   Article

   Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

   Insert

   SUZ12 (SUZ12 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

   Plasmid

   #181791

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLib6.4B-Consensus

   Plasmid

   #176678

   Purpose

   Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

   Plasmid

   #181790

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRepair-MPP8_mAID-P2A-BFP

   Plasmid

   #175036

   Purpose

   Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

   Depositor

   Kristian Helin

   Article

   Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

   Insert

   mAID-P2A-BFP
   

   Use

   Hdr donor template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

   Plasmid

   #181789

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLib6.4-Aalb_743

   Plasmid

   #176662

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p944-SWITCH-OVER insert: EF1a-Puro

   Plasmid

   #217891

   Purpose

   insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

   Plasmid

   #181788

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEasyG1_hph

    Plasmid

    #184912

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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