We narrowed to 9,663 results for: BLI

1. PX383 Lactobacillus johnsonii Cas9

   Plasmid

   #68320

   Purpose

   Lactobacillus johnsonii Cas9

   Depositor

   Feng Zhang

   Insert

   LjCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-3'TwStrep

   Plasmid

   #208618

   Purpose

   Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   Twin Strep

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX386 Lactobacillus farciminis Cas9

   Plasmid

   #68323

   Purpose

   Lactobacillus farciminis Cas9

   Depositor

   Feng Zhang

   Insert

   LfCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.3_SARS2_XBB.1.5

   Plasmid

   #196585

   Purpose

   expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (XBB.1.5)
   

   Expression

   Mammalian

   Mutation

   G252V, F486P on the basis of XBB

   Promoter

   CMV

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXR003_puroR

   Plasmid

   #219819

   Purpose

   hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7TS-rich

   Plasmid

   #99050

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Use

   Unspecified; Xenopus oocyte system

   Promoter

   pT7

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIP4

   Plasmid

   #233686

   Purpose

   Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.

   Depositor

   Christopher Bahl

   Insert

   Smt3 (SMT3 )
   

   Tags

   HisTag

   Promoter

   T7

   Available Since

   April 7, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. LentiCROP-PuroR-P2A-GFP-U6

   Plasmid

   #200029

   Purpose

   Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

   Depositor

   Jay Shendure

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   PuroR-P2A-GFP

   Expression

   Mammalian

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

   Plasmid

   #219826

   Purpose

   Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 29, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLenti6.2_mCherry_roGFP2_Orp1

    Plasmid

    #155043

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGs-Venus

    Plasmid

    #127346

    Purpose

    Expresses Venus constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Venus Fluorescent Protein
    

    Expression

    Mammalian

    Promoter

    CAGGS Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJM105: pTac RFP on pCM66T

    Plasmid

    #74746

    Purpose

    pCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)

    Depositor

    Mary Lidstrom

    Insert

    RFP (BioBrick part number BBa_E1010)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pTac

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hsp68-LacZ-Gibson

    Plasmid

    #170102

    Purpose

    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.

    Depositor

    Len Pennacchio

    Type

    Empty backbone

    Use

    Mouse Targeting

    Promoter

    Mouse Hsp68 minimal promoter

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-29b(+)_His-CBD-3C-EmPNGaseF

    Plasmid

    #218193

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF

    Depositor

    Florence Pojer

    Insert

    PNGaseF
    

    Tags

    tufA-His-CBD-3C

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 41-354

    Promoter

    T7

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. NNMT

    Plasmid

    #40734

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NNMT (NNMT Human)
    

    Tags

    His Thrombin

    Expression

    Bacterial

    Mutation

    K100A:E101A:E103A

    Available Since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pBS L30 mRuby3-Rab27A

    Plasmid

    #166432

    Purpose

    Rab27A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab27A
    

    Tags

    mRuby3

    Expression

    Mammalian

    Promoter

    L30 weak promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. NOVA1 eGFP

    Plasmid

    #61275

    Purpose

    GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    NOVA1 (NOVA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

    Plasmid

    #28289

    Purpose

    3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)

    Depositor

    Gus Frangou, Alex Chenchik

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV6-LacZ-BbsI

    Plasmid

    #114671

    Purpose

    For one-step cloning (with blue/ white screening) of an open reading frame of interest for constitutive gene expression

    Depositor

    Lynne Postovit

    Insert

    LacZ
    

    Expression

    Mammalian

    Promoter

    Lac promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-Flag-hS1pr1_Neo

    Plasmid

    #203709

    Purpose

    Overexpression of FLAG-S1PR1.

    Depositor

    Timothy Hla

    Insert

    Flag-hS1PR1 (S1PR1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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