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IF701: pMVP (L5-L4) APEX2

Plasmid

#121725

Purpose

pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

APEX2 (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IG301: pMVP (L1-R5) CMV promoter

Plasmid

#121692

Purpose

pMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3C G126

Plasmid

#123079

Purpose

Gateway entry clone encoding human MAP1LC3C G126

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway entry vector / entry clone

Mutation

Deleted amino acids 127-147. Stop codon after G12…

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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IZ401: pMVP/Lenti/Neo-DEST

Plasmid

#121850

Purpose

pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK001: pMVP/SB/Hygro-DEST

Plasmid

#121867

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KTK_136

Plasmid

#180520

Purpose

Empty backbone for D1.1 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP no-stop

Plasmid

#123209

Purpose

Gateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway entry vector / entry clone

Mutation

Stop codon removed

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3C no-stop

Plasmid

#123208

Purpose

Gateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway entry vector / entry clone

Mutation

Stop codon removed

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL1 G116A

Plasmid

#123084

Purpose

Gateway entry clone encoding human GABARAPL1 G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway entry vector / entry clone

Mutation

Glycine 116 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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HE401: pMVP (L1-R5) shRNA

Plasmid

#121698

Purpose

pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj11

Plasmid

#173139

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj11 (kcnj11 Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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JZ901: pMVP (L5-L4) EF1a promoter

Plasmid

#121727

Purpose

pMVP L5-L4 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_B2519

Plasmid

#91076

Purpose

Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

OsU3

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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IG001: pMVP (L1-L4) TBG promoter

Plasmid

#121689

Purpose

pMVP L1-L4 entry plasmid, contains TBG promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TBG promoter (Liver specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT504: pMVP/SB/eGFP-DEST

Plasmid

#121861

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT601: pMVP/SB/mCherry-DEST

Plasmid

#121862

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LF601: pMVP (L1-R5) TBG promoter

Plasmid

#121695

Purpose

pMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TBG promoter (Liver specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-mouse TCR zeta/2451

Plasmid

#11506

Depositor

Allan Weissman

Article

Weissman et al Science. 1988 Feb 26. 239(4843):1018-21.

Insert

TCR zeta (Cd247 Mouse)

Use

General cloning

Mutation

n/a

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

Plasmid

#140200

Purpose

Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

dLbCas12a crRNA scaffold

Promoter

PgpdA

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(PLF)-HA no-pA

Plasmid

#82597

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

K2095P, T2098L, W2101F

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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