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pDONR221_SV2A

Plasmid

#132257

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SV2A (SV2A Human)

Expression

Mammalian

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221_SLC5A2

Plasmid

#132241

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC5A2 (SLC5A2 Human)

Expression

Mammalian

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pDONR221_SLC16A1

Plasmid

#131934

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A1 (SLC16A1 Human)

Expression

Mammalian

Available since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

Plasmid

#113694

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Publication

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR221_SLC16A3

Plasmid

#131899

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A3 (SLC16A3 Human)

Expression

Mammalian

Available since

Oct. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pDONR221_SLC2A3

Plasmid

#132114

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A3 (SLC2A3 Human)

Expression

Mammalian

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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FASTHDR-Cterm-mClover3-Puromycin

Plasmid

#167205

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

Depositor

Oscar Perez

Publication

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mClover3

Expression

Mammalian

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC6A19

Plasmid

#132231

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A19 (SLC6A19 Human)

Expression

Mammalian

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pLenti_PE7-2A-BSD

Plasmid

#234073

Purpose

For lentiviral expression of PE7 with blasticidin resistance

Depositor

Greg Findlay

Publication

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PE7

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#194703

Purpose

Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

Depositor

Eugene Yeo

Publication

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Tags

APOBEC1-HA-P2A-mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-hKif5b(1-560)rigor-2xmNeongreen

Plasmid

#174649

Purpose

Live-cell marker for stable microtubules

Depositor

Lukas Kapitein

Publication

Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

Insert

hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)

Tags

mNeongreen-mNeongreen

Expression

Mammalian

Mutation

rigor mutation G234A

Promoter

Chicken beta-actin

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Publication

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Expression

Bacterial

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Publication

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

W4 (modified T7A1)

Available since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

Plasmid

#127354

Purpose

gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Publication

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and mVenus

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Publication

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Expression

Mammalian

Promoter

CMV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-BI

Plasmid

#126475

Purpose

High-copy number vector for the co-expression of two genes in mammalian cells

Depositor

Bill Hansson

Publication

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3

Plasmid

#116735

Purpose

Lentiviral expression of ERBB3

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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EasyFusion Halo

Plasmid

#112850

Purpose

General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

HaloTag

Use

Synthetic Biology

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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