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YFP-HA-DAT W63A

Plasmid

#90246

Purpose

Expresses YFP-HA-DAT W63A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated W63 to A

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT R60A

Plasmid

#90245

Purpose

Expresses YFP-HA-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated R60 to A

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:FKBP

Plasmid

#135984

Purpose

Expresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the FKBP domain

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTW063

Plasmid

#115950

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 SGP (+/- Apt) Dest

Use

Synthetic Biology

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA3.8.3

Plasmid

#121959

Purpose

Mammalian expression, Transcription regulation, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, MS2 hairpin inserted

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA_(1,20,6)-GFP

Plasmid

#124619

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(1,20,6)

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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GWF303

Plasmid

#133611

Purpose

CD95-1-2xPP7, NLS-PCP-DNCR2-P2a-BFP

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

CD95-1-2xPP7, PCP-DNCR2, BFP

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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GWF314

Plasmid

#133610

Purpose

CXCR4-com, NLS-com-GNCR1-P2a-BFP

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

CXCR4-6-com, com-GNCR1, BFP

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-Raf1RBD

Plasmid

#120707

Purpose

Expresses Flag-HA-6xHIS-Raf1 RBD fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Raf1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Raf1 amino acids 1-149

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTW322

Plasmid

#115953

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P1 With 3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW323

Plasmid

#115954

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P1 No 3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW325

Plasmid

#115956

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P2 With 3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW326

Plasmid

#115957

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P2 No 3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW327

Plasmid

#115958

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P2 End

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW328

Plasmid

#115959

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P3 With E1-3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4 rat NZAP

Plasmid

#45908

Depositor

Harmit Malik

Article

Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

Insert

Zc3hav1 zinc finger CCCH type, antiviral 1 (Zc3hav1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only
pMCh-1468

Plasmid

#82418

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis GW182 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA M4

Plasmid

#80937

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR-PTPRJi1

Plasmid

#59608

Purpose

Expression of shRNA against human PTPRJ

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPRJ (PTPRJ Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM21i2

Plasmid

#59605

Purpose

Expression of shRNA against human ADAM21

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM21 (ADAM21 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only