We narrowed to 69,454 results for: nin
-
Plasmid#178055Purposemyc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.DepositorInsertToll-Interacting Protein (TOLLIP Human)
TagsmycBioIDExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1-4H pET151
Plasmid#159386PurposeExpresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 (TLNRD1 Human)
Tagshis-tag, TEV cleavage siteExpressionBacterialMutationTLNRD1 4-helix domain (residues 143-273)PromoterT7Available SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge_RtoK
Plasmid#139127Purposeexpress FUS LC with hnRNPA2-like charge patterning where all R are changed to KDepositorInsertFUS (FUS Human)
ExpressionBacterialMutationAdd K and D to FUS LC so it has the same charge p…Available SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS
Plasmid#153445PurposeE. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
Tags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG). Argi…PromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_E110A/pET28b(+) N-tag
Plasmid#71354Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:E110ADepositorInsertHP0518 (HP0518 changed Glutamic acid 110 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_Y133A/pET28b(+) N-tag
Plasmid#71356Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:Y133ADepositorInsertHP0518 (HP0518 changed Tyrosine 133 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_C176A/pET28b(+) N-tag
Plasmid#71360Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:C176ADepositorInsertHP0518 (HP0518 changed Cysteine 176 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_H160A/pET28b(+) N-tag
Plasmid#71359Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:H160ADepositorInsertHP0518 (HP0518 changed Histidine 160 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_W158A/pET28b(+) N-tag
Plasmid#71358Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:W158ADepositorInsertHP0518 (HP0518 changed Tryptophan 158 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_H155A/pET28b(+) N-tag
Plasmid#71357Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:H155ADepositorInsertHP0518 (HP0518 changed Histidine 155 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_Y132A/pET28b(+) N-tag
Plasmid#71355Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:Y132ADepositorInsertHP0518 (HP0518 changed Tyrosine 132 to Alanine)
TagsHistidine tagExpressionBacterialPromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cyt-n
Plasmid#53065Purposedestination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-er-n
Plasmid#53067Purposedestination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-tgn-n
Plasmid#53071Purposedestination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
AspFlex Kit
Plasmid Kit#1000000217PurposeThis collection is a set of Modular Cloning (MoClo) plasmids for Acinetobacter spp. and Acinetobacter baumannii for protein expression analyses and CRISPRi experiments.DepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Endy/Shearwin pOSIP plasmid set
Plasmid Kit#1000000035Purpose11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elementsDepositorAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
CXCR5-DuET
Plasmid#213222PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR7-DuET
Plasmid#213203PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR156-DuET
Plasmid#213267PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only