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We narrowed to 15,513 results for: otos

Showing: 3281 - 3300 of 15513 results

  1. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid
    #69534
    Purpose
    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
    Depositor
    Timo Otonkoski
    Article
    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5
    Insert
    DD-dCas9VP192-T2A-EGFP
    Use
    Tags
    C-term T2A-EGFP and DHFR Destabilised Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69534-plasmid-map-sequence-id-122831

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  2. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available Since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

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  5. pcpAzpaRS_v2

    Plasmid
    #196483
    Purpose
    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196483-plasmid-map-sequence-id-388871

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  6. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102885
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102885-plasmid-map-sequence-id-259794

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  11. pJS288

    Plasmid
    #200714
    Purpose
    To express yeast Hxt1 hexose transporter in yeast
    Depositor
    Jens Boch
    Article
    Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi
    Inserts
    PMA1 promoter (PMA1 Budding Yeast)
    Hxt1 (HXT1 Budding Yeast)
    ADH2 Ter (ADH2 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    domesticated for MoClo cloning (removal of intern…
    Promoter
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    200714-plasmid-map-sequence-id-404927

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Showing: 3281 - 3300 of 15513 results