We narrowed to 1,697 results for: lin tian

1. pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

   Plasmid

   #182354

   Purpose

   Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

   Plasmid

   #182356

   Purpose

   Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE30-DARPin-E11

   Plasmid

   #226784

   Purpose

   Expression of His-tagged DARPin E11 in E. coli

   Depositor

   Sebastian Mathea

   Article

   Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

   Insert

   DARPin E11
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTSK405

   Plasmid

   #190817

   Purpose

   For C-terminus 3xGFP tagging of a gene of interest using URA3 selection marker in S. cerevisiae

   Depositor

   Tatiana Karpova, Gunjan Mehta

   Article

   Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

   Insert

   URA3 (URA3 Budding Yeast)
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEM266 - pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

   Plasmid

   #159829

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

   Plasmid

   #159833

   Purpose

   Single copy or array insertion by MosTI using split NeoR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBKWH-p53

   Plasmid

   #133900

   Purpose

   Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   p53 (Trp53 Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   T7

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1_SPTLC1_C133W

   Plasmid

   #181921

   Purpose

   3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W

   Depositor

   Christian Metallo

   Article

   Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

   Insert

   Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
   

   Use

   Lentiviral; Mammalian expression, , doxycycline i…

   Tags

   Expression

   Mammalian

   Mutation

   cysteine at position 133 of the coding region of …

   Promoter

   tight TRE promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBSWHhc-p53

   Plasmid

   #133903

   Purpose

   Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   p53 (TP53 Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   T7

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Myc-NLRP3(R260W)

    Plasmid

    #73956

    Purpose

    Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    R260W

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only