We narrowed to 6,241 results for: nls

1. Lentiviral-S-tdTomato

   Plasmid

   #112579

   Purpose

   Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

   Depositor

   Jeffrey Rothstein

   Article

   Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

   Insert

   NLS-tdTomato-NES
   

   Use

   Lentiviral

   Tags

   nuclear export sequence and nuclear localization …

   Expression

   Mammalian

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

   Plasmid

   #188778

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   EF1a

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. hOT-GFP

   Plasmid

   #67848

   Purpose

   mammalian expression of human oxytocin receptor with GFP tag

   Depositor

   Christian Gruber

   Article

   Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

   Insert

   oxytocin receptor (OXTR Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-Ucp1-Cre-miR122

   Plasmid

   #228408

   Purpose

   Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

   Depositor

   Joerg Heeren

   Article

   Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

   Inserts

   cre recombinase with SV40 NLS
   Cre recombinase with SV40 NLS
   

   Use

   AAV

   Promoter

   rat Ucp1 enhancer-promoter

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

   Plasmid

   #188767

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-GFP/Cre

   Plasmid

   #49056

   Purpose

   Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

   Depositor

   Fred Gage

   Article

   Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

   Insert

   Cre
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   EGFP, Myc, and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-fDIO-Cre

   Plasmid

   #121675

   Purpose

   Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

   Depositor

   Alexander Nectow, Esteban Engel

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Has Service

   AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

   Insert

   NLS-CRE-HA
   

   Use

   AAV

   Tags

   3xHA and SV40-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TDP-43_NTD1-80_E17R

   Plasmid

   #104479

   Purpose

   bacterial expression of TDP-43 NTD E17R

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   E17R

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

   Plasmid

   #188779

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBridge-tyrosinase.σ2

    Plasmid

    #197415

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-2 σ2
    

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    encodes R42G substitution, contains silent substi…

    Promoter

    ADH1 and MET25

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEvol-pAzFRS.1.t1

    Plasmid

    #73547

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.1.t1
    pAzFRS.1.t1
    

    Expression

    Bacterial

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188773

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-UCOE-EF1a-Zim3-dCas9-BFP

    Plasmid

    #188775

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9-mTagBFP2
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDRM166 LKB (Luciferase mKate2 Blast)

    Plasmid

    #183502

    Purpose

    Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    mKate2
    BSD
    

    Use

    Lentiviral and Luciferase

    Tags

    P2A linker and SV40 NLS with GGS linker

    Expression

    Mammalian

    Promoter

    MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-PEmax SpG

    Plasmid

    #187895

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax, SpG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-UCOE-SFFV-Zim3-dCas9-BFP

    Plasmid

    #188776

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9-mTagBFP2
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C1-AR Q48

    Plasmid

    #86428

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Poly-glutamine expansion, G130D

    Promoter

    CMV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CMVc-Cas9

    Plasmid

    #106431

    Purpose

    Expresses SpCas9 from the CMVc promoter in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Promoter

    CMVc

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MBP-FUS_LC_WT

    Plasmid

    #98653

    Purpose

    Expresses MBP-tagged FUS AA 1-163

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-AR Q0

    Plasmid

    #86427

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    androgen receptor (AR) (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the poly-glutamine expansion

    Promoter

    CMV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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