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pLX311_CdTag-HA_EmptyVector

Plasmid

#221468

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE-EGFP

Plasmid

#36999

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pARE-lux

Plasmid

#11768

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

ARE

Use

Luciferase

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP×3-N1

Plasmid

#86776

Purpose

A mammalian expression vector with triple EGFP at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pBJ5-CnA

Plasmid

#11785

Depositor

Anjana Rao

Article

Clipstone et al J Biol Chem. 1994 Oct 21. 269(42):26431-

Insert

CnA (Ppp3ca Mouse)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pGWT35S

Plasmid

#182790

Purpose

Destination vector for transient expression in plant

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Type

Empty backbone

Expression

Plant

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAG384 bFos-CFAST11

Plasmid

#130815

Purpose

Expresses bFos-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

bFos-CFAST11

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBS hBAF155

Plasmid

#17876

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF155 (SMARCC1 Human)

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pNIC- AVITAG-CTH10STII- -GG

Plasmid

#215805

Purpose

Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMAK461

Plasmid

#140699

Purpose

NBαRb-IgG-Fc

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαRb-IgG-Fc

Expression

Bacterial

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET-NDH6

Plasmid

#105503

Purpose

Expression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tag

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

NDH6

Tags

6xHis

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-hBAF60a

Plasmid

#21034

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF60a (SMARCD1 Human)

Use

Phagemid

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pCB301TOR

Plasmid

#164798

Purpose

Oomycete expression vector using Agrobacterium-mediated transformation

Depositor

Miaoying Tian

Article

Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

Insert

NPTII

Use

Oomycete expression

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-hBAF60c

Plasmid

#21036

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF60c (SMARCD3 Human)

Use

Phagemid

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pKDM

Plasmid

#110740

Purpose

Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pYS2

Plasmid

#117490

Purpose

Generation of unmarked gene deletions in Mycobacterium smegmatis

Depositor

Eyal Gur

Article

Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

Insert

gfp, hyg

Use

Deletion generation

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHA-N1

Plasmid

#86049

Purpose

A mammalian expression vector with a HA-tag at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pPGS-3HA-TEAD1

Plasmid

#33055

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD1 (TEAD1 Human)

Tags

3HA

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pAc5.1C-FLuc-Stop-5BoxB

Plasmid

#21301

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

5BoxB

Use

Drosophila expression

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pBS-hBAF60b

Plasmid

#21035

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF60b (SMARCD2 Human)

Use

Phagemid

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pLX307_CdTag-HA_EmptyVector

Plasmid

#221467

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMS2-1

Plasmid

#220627

Purpose

Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS6-GST-TEV-GG

Plasmid

#215801

Purpose

Cloning of target gene with N terminal His6 GST tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMAK462

Plasmid

#140700

Purpose

NBαMs/Rt-IgG1-Fc

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαMs/Rt-IgG1-Fc

Expression

Bacterial

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-LSSmCherry1(-13)

Plasmid

#219415

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

LSSmCherry1(-13)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

K137E, K199E

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAPA3

Plasmid

#24777

Depositor

Tanya Parish

Article

Parish et al J Bacteriol. 2007 May . 189(10):3721-8.

Type

Empty backbone

Use

Integrating vector

Expression

Bacterial

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 4x

Plasmid

#20930

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Insert

4x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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C054

Plasmid

#174342

Purpose

Template for making Fluc mRNA reporter with pA124 and mutant mouse Malat1.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Firefly luciferase

Use

In vitro transcription template

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS6-THRX-TEV-GG

Plasmid

#215796

Purpose

Cloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBRIT TAP

Plasmid

#17520

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

TAP

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
hHpr1-GST

Plasmid

#11200

Depositor

Robin Reed

Article

Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

Insert

Hpr1 (THOC1 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pLX302_CdTag-HA_EmptyVector

Plasmid

#221465

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIC-NHSTIIT-AVITAG-GG

Plasmid

#215802

Purpose

Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMLS257

Plasmid

#73716

Purpose

SapTrap destination vector for building repair template only vectors

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SapI repair template insertion site

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsPAN2_M

Plasmid

#146941

Purpose

Mammalian Expression of HsPAN2

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsPAN2 (PAN2 Human)

Expression

Mammalian

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAK463

Plasmid

#140701

Purpose

NBαMs-IgK

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαMs-IgK

Expression

Bacterial

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS hBAF170

Plasmid

#17875

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF170 (SMARCC2 Human)

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
OR gate

Plasmid

#44451

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

OR-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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Endophilin SH3 domains

Plasmid

#47401

Depositor

Peter McPherson

Article

Micheva et al J Biol Chem. 1997 Oct 24;272(43):27239-4

Insert

Endophilin

Tags

GST

Expression

Bacterial

Mutation

SH3 domain (aa 292-352)

Promoter

tac

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pBsaGSCYP101A1

Plasmid

#108206

Purpose

CYP101A1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

CYP101A1

Expression

Bacterial

Promoter

lac promoter

Available Since

April 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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