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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,356 results for: BASE

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Showing: 3321 - 3340 of 13356 results

  3. PB-sgRNA(MS2)

    Plasmid
    #102560
    Purpose
    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102560-plasmid-map-sequence-id-198595

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  5. pET-APOBEC1-YTH

    Plasmid
    #200158
    Purpose
    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification
    Depositor
    Kate Meyer
    Article
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Insert
    APOBEC1-YTH
    Tags
    6xHis, HA, and Maltose Binding Protein
    Expression
    Bacterial
    Mutation
    Codon optimized for E coli expression
    Promoter
    LacZ
    Available Since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200158-plasmid-map-sequence-id-396323

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  9. pHR-hSyn-EGFP

    Plasmid
    #114215
    Purpose
    Lentiviral vector (control) that targets expression of EGFP to neurons
    Depositor
    Xue Han
    Article
    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Lentiviral and Mouse Targeting
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
    Promoter
    human synapsin (hSyn)
    Available Since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    114215-plasmid-map-sequence-id-223405

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  12. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  13. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid
    #185913
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185913-plasmid-map-sequence-id-367365

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Showing: 3321 - 3340 of 13356 results