We narrowed to 12,472 results for: BASE

1. qTAG-N-Zeo-mNeon

   Plasmid

   #207698

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mNeon cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Puro-V5

   Plasmid

   #207697

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. qTAG-N-Blast-moxGFP

   Plasmid

   #207677

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. mAzurite-8xbiRhoBAST-24xMS2v6

   Plasmid

   #196352

   Purpose

   Expression of mAzurite mRNA with 8xbiRhoBAST-24xMS2v6 in 3'UTR

   Depositor

   Murat Sunbul

   Article

   Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

   Insert

   mAzurite-8xbiRhoBAST-24xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Zeo-mScarlet

   Plasmid

   #207700

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mScarlet cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. qTAG-N-Zeo-dTAG

   Plasmid

   #207705

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. AAV-Synapsin-mCherry-3XNLS-WPRE

   Plasmid

   #159951

   Purpose

   nucleus marker

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mCherry-3XNLS
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MazF-WT

   Plasmid

   #155202

   Purpose

   To express recombinant MazF protein.

   Depositor

   Guanzheng Luo

   Article

   Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

   Insert

   MazF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-miRFP670nano3-Blast

   Plasmid

   #207713

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pMK465 (mAB-Hygro)

    Plasmid

    #214391

    Purpose

    mAID-BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    mAID-BrpmpTag-Hygro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. qTAG-C-V5-Zeo

    Plasmid

    #207741

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pChimera

    Plasmid

    #61476

    Purpose

    Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    U6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0090

    Plasmid

    #181954

    Purpose

    Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    7xTOPFlash
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJH136

    Plasmid

    #47554

    Purpose

    pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

    Insert

    URA3
    

    Use

    Pcr template

    Tags

    Expression

    Mutation

    Promoter

    Native URA3 promoter, -243 to -1.

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAS9-TPC

    Plasmid

    #61478

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PcUbi4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pN-PITCh-GFP

    Plasmid

    #127888

    Purpose

    Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    Puro-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-CRY2-mCh-superPLDx30

    Plasmid

    #188988

    Purpose

    A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CRY2 and mCherry

    Expression

    Mammalian

    Mutation

    K109R, P245A, G328S, G381V, G429D

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-cMSP1D1

    Plasmid

    #199768

    Purpose

    Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP1D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFUS_B4

    Plasmid

    #31021

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. ALIX-His(1-868, P801G)

    Plasmid

    #186808

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    ALIX-His (1-868, P801G) (PDCD6IP Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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