We narrowed to 25,468 results for: FER

1. pAAV/hSyn-SCLM

   Plasmid

   #216760

   Purpose

   AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon

   Depositor

   George Augustine

   Insert

   SuperClomeleon
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S30R and 11 AA deletion in the Cerulean CFP moiet…

   Promoter

   hSyn (human synapsin I)

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1-H-Luc_cyto

   Plasmid

   #113929

   Purpose

   Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Spacer Switch Plasmid

   Plasmid

   #215346

   Purpose

   Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

   Depositor

   Hank Seifert

   Article

   Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

   Insert

   OpaD spacer; Chloramphenicol Resistance Cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL6

   Plasmid

   #48746

   Purpose

   Expression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   mPer2 promoter/enhancer region (-1128 to +2129)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

5. Omega Destination-CMV::ELuc:bGH

   Plasmid

   #124528

   Purpose

   Omega destination vector, that has a CMV:ELUC:bgH in the backbone

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Type

   Empty backbone

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK2-2A-3xFLAG-XBP1u_pause-2A

   Plasmid

   #159583

   Purpose

   Luciferase reporter for ribosome pausing

   Depositor

   Shintaro Iwasaki

   Article

   Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

   Inserts

   XBP1u pause site
   EIF2S3 5UTR
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas9-J23111

   Plasmid

   #113149

   Purpose

   Plasmid constitutively expressing dCas9 protein

   Depositor

   Chong Zhang

   Article

   Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A & H840A

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.R_TRE_minPro

   Plasmid

   #211517

   Purpose

   Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

   Depositor

   Justin English

   Article

   Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SV40 and minPro

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-PUb

   Plasmid

   #52891

   Purpose

   Aedes aegypti polyubiquitin promoter driving firefly luciferase

   Depositor

   Zach Adelman

   Article

   Anderson et al Insect Mol Biol. 2010 Aug;19(4):441-9. d

   Insert

   polyubiquitin promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Ae aegypti polyubiquitin

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-puro-Flag-mMcl-1

    Plasmid

    #32982

    Depositor

    Joseph Opferman

    Article

    Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

    Insert

    Mcl-1 (Mcl1 Mouse)
    

    Use

    Retroviral

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. 2xMARS-nGFP

    Plasmid

    #205235

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSV2neo human IFN-gamma

    Plasmid

    #10954

    Depositor

    Howard Young

    Article

    Young et al J Immunol. 1986 Jun 15. 136(12):4700-3.

    Insert

    interferon-gamma (IFNG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

13. punc-119c

    Plasmid

    #21595

    Depositor

    Al Fisher

    Article

    Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

    Insert

    unc-119 (unc-119 Nematode)
    

    Use

    Adds unc-119 marker to amp resistant plasmids

    Tags

    Expression

    Worm

    Mutation

    N/A

    Promoter

    Available since

    Aug. 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBPGAL4.2::p65Uw

    Plasmid

    #26229

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL4::p65 (RELA Budding Yeast, Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1-S-Luc_cyto

    Plasmid

    #113926

    Purpose

    Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKERep

    Plasmid

    #211826

    Purpose

    Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKESa

    Plasmid

    #211828

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. hum sgk promoter F4R4

    Plasmid

    #83426

    Purpose

    promoter reporter construct to test 5' flanking sequences

    Depositor

    Christie Thomas

    Article

    Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

    Insert

    human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    sgk

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a(+)- PC-MjMAT

    Plasmid

    #179372

    Purpose

    PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

    Depositor

    Andrea Rentmeister

    Article

    Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

    Insert

    PC-MjMAT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L147A, I351A

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4.R_TRE_minTK

    Plasmid

    #211516

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SV40 and minTK

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge