We narrowed to 150,505 results for: ins

1. pcDNA3.1(+)_Granulin 4+linker5

   Plasmid

   #176924

   Purpose

   express Granulin 4 with linker 5 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Granulin 4+linker5 (GRN Human)
   

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

   Plasmid

   #133793

   Purpose

   U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   CjeCas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   V2-TGAA linker sgRNA architecture from Kim et al.…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

   Plasmid

   #104569

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-DmrA(x2)
   

   Tags

   3x FLAG, 3x HA, and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST40-2XHA-Src-S32

   Plasmid

   #140308

   Purpose

   Expresses HA-tagged human Src-S32 in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   contains only the SH3 and SH2 domains

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. JA524

   Plasmid

   #49938

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in EGFP for use as a control

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD GFP (TET1 Human)
   

   Use

   TALEN

   Promoter

   EF1a

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAAV_ACTB CRISPIE

   Plasmid

   #172853

   Purpose

   Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Inserts

   Actb intron 1 sgRNA
   DRS-2 sgRNA
   CRISPIE designer exon (phase 0-0) encoding mEGFP
   mRuby3
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MLM3727

   Plasmid

   #49961

   Purpose

   Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x Flag Tet1CD HB-5 (HBB Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Promoter

   EF1a

   Available Since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

8. VC388

   Plasmid

   #49958

   Purpose

   Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x Flag Tet1CD HB-4 (HBB Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Promoter

   EF1a

   Available Since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pJW1927

   Plasmid

   #163093

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SL536

    Plasmid

    #49942

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

11. JA1246

    Plasmid

    #49960

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-5 (HBB Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

12. LZF40: LZF40_Fsyn_Cre

    Plasmid

    #109378

    Purpose

    Synaptic transmission

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    Cre-NCBI
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    human synapsin I

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. JA1238

    Plasmid

    #49957

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-4 (HBB Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

14. MLM3733

    Plasmid

    #49964

    Purpose

    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CD HB-6 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Promoter

    EF1a

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

15. SL539

    Plasmid

    #49948

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

16. JA1261

    Plasmid

    #49963

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-6 (HBB Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

17. JA1290

    Plasmid

    #49968

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-9 (HBB Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

18. JA1252

    Plasmid

    #49956

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD HB-3 (HBB Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pMSCV-FKBP-GMIP(pLxIS)

    Plasmid

    #221270

    Purpose

    Expression of GMIP pLxIS (1-84) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    GMIP pLxIS (1-84) (Gmip Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid

    #222876

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FRB chain: V84R in GPA

    Promoter

    MSCV LTR

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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