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We narrowed to 3,533 results for: mpo

Showing: 3321 - 3340 of 3533 results

  4. LiPOP2

    Plasmid
    #168266
    Purpose
    A single-component optogenetic tool for photoswitchable pyroptosis
    Depositor
    Yubin Zhou
    Article
    He et al Adv Sci (Weinh). 2021 May 6;8(13):210042
    Insert
    mGSDMD(1-276) (Gsdmd Mouse)
    Tags
    cpLOV2, mCherry
    Expression
    Mammalian
    Mutation
    R138A, K146A, R152A, R154A
    Promoter
    CMV
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168266-plasmid-map-sequence-id-334500

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  7. pBS-AMVpl-β1WT-nAchR

    Plasmid
    #97080
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Beta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97080-plasmid-map-sequence-id-189664

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  11. PEA1

    Plasmid
    #176527
    Purpose
    Delivers all prime editing nickase components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available Since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176527-plasmid-map-sequence-id-350558

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  14. JE301: pMVP (L5-L4) Tir1-P2A-AID

    Plasmid
    #121722
    Purpose
    pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    osTir1-P2A-AID (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    9x myc (C-term on osTIR1)
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121722-plasmid-map-sequence-id-237078

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  15. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47455
    Purpose
    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    Use
    AAV
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47455-plasmid-map-sequence-id-69627

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  16. pET24a-VHH-tev-std

    Plasmid
    #109423
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109423-plasmid-map-sequence-id-260701

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  17. KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

    Plasmid
    #121831
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    x-dCas9(3.7)/LSD1 (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121831-plasmid-map-sequence-id-237385

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  19. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid
    #121833
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    x-dCas9(3.7)/Dnmt3a-3L (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121833-plasmid-map-sequence-id-237387

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  20. IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA

    Plasmid
    #121799
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + CMV::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121799-plasmid-map-sequence-id-237303

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Showing: 3321 - 3340 of 3533 results