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FH1-PNGT#2-mCherry

Plasmid

#164099

Purpose

Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

PNGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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FH1-MGT#2-mCherry

Plasmid

#164097

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_eCB2.0mut

Plasmid

#164607

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNUAK1

Plasmid

#138692

Purpose

Expresses a human NUAK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNUAK1 human (NUAK1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNUAK2

Plasmid

#138693

Purpose

Expresses a human NUAK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNUAK2 human (NUAK2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLex_Cas9

Plasmid

#117987

Purpose

pLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter.

Depositor

Paul Khavari

Article

Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

Insert

Cas9-P2A-NLS-BASTR

Use

Lentiviral

Tags

P2A linker, nuclear localization signal and blast…

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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anti-Desmin_D7-pBIOCAM5

Plasmid

#39346

Depositor

John McCafferty

Article

Schofield et al Genome Biol. 2007;8(11):R254.

Insert

scFv-Fc-fusion specific for Desmin (clone D7) (DES Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLXSN p110 CUX1

Plasmid

#90471

Purpose

Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively

Depositor

Alain Nepveu

Article

Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

Insert

CUX1 (amino acids 747-1505) (CUX1 Human)

Use

Retroviral

Tags

HA and Myc

Expression

Mammalian

Promoter

Moloney murine leukemia virus long terminal repeat

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78603

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3/hArf5(WT)-mCherry

Plasmid

#79421

Purpose

Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf5 (ARF5 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-E2F2 3'UTR

Plasmid

#29469

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

E2F2 3'UTR (E2F2 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

Plasmid

#223959

Purpose

Dual fluorescent reporter for histone H2B and for APC/C activity

Depositor

Hee Won Yang

Article

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)

Use

Lentiviral

Tags

iRFP670 and mCerulean

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-T-GECO1

Plasmid

#191616

Purpose

Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

Depositor

Robert Campbell

Article

Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

Insert

T-GECO1

Tags

His tag

Expression

Bacterial

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-C SARS-CoV-2 Omicron spike protein

Plasmid

#180778

Purpose

Sars-CoV-2 variant protein

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

MAC-C SARS-CoV-2 Omicron spike protein

Tags

MAC-tag

Expression

Mammalian

Mutation

C64W , S371L, H69-V70-, S373P, S375F, G339D, N211…

Available Since

April 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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evoCas9

Plasmid

#138558

Purpose

Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

evoCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

M495V, Y515N, K526E, R661Q

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CreLite

Plasmid

#131785

Purpose

AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

AAV, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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MTK1_008

Plasmid

#123671

Purpose

Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC7A3

Plasmid

#132301

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

Insert

SLC7A3 (SLC7A3 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pRAB18-GFP

Plasmid

#106984

Purpose

ABA signaling reporter, GFP under the RAB18 promoter

Depositor

Julian Schroeder

Article

Kim et al Curr Biol. 2011 Jun 7;21(11):990-7. doi:

Insert

sGFP

Expression

Plant

Promoter

RAB18

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_ORI_IGF1R

Plasmid

#99306

Purpose

Luciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

enhancer; chr15: 99439352 -99440791

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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