We narrowed to 11,355 results for: SHA

1. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

   Plasmid

   #62581

   Purpose

   Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

   Promoter

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.2-Cx43-M100/125/147L-siResist

   Plasmid

   #49856

   Purpose

   Encodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNA

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Methionine100, Methionine 125, and Methionine 147…

   Promoter

   CMV

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.2-Cx43-M100/125L-siResist

   Plasmid

   #49855

   Purpose

   Encodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Methionine100 and Methionine 125 mutated to Leuci…

   Promoter

   CMV

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pNK3071

   Plasmid

   #219755

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNK497

   Plasmid

   #219754

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-flex-taCasp3-TEVp

   Plasmid

   #45580

   Depositor

   Jim Wells, Nirao Shah

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Has Service

   AAV5

   Inserts

   Caspase 3 (CASP3 Human, Synthetic)
   Tobacco Etch Virus protease
   

   Use

   AAV; Adeno associated viral vector

   Tags

   Expression

   Mutation

   Linker replaced with a TEV protease cleavage site

   Promoter

   EF1a

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP

   Plasmid

   #158654

   Purpose

   making transgenics expressing a QUAS driven YFP and NTR 2.0

   Depositor

   Jeff Mumm

   Article

   Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

   Insert

   5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP
   

   Use

   Tol2-based transgenic expression in zebrafish/oth…

   Tags

   Expression

   Mutation

   F70A/F108Y

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

   Plasmid

   #158653

   Purpose

   making transgenics expressing a UAS driven mCherry and NTR 2.0

   Depositor

   Jeff Mumm

   Article

   Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

   Insert

   5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
   

   Use

   Tol2-based transgenic expression in zebrafish/oth…

   Tags

   Expression

   Mutation

   F70A/F108Y

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FUW-hSNCA-GFP

   Plasmid

   #215489

   Purpose

   Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

   Depositor

   Ophir Shalem

   Article

   Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158652

    Purpose

    making transgenics expressing a UAS driven eGFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Tags

    Expression

    Mutation

    F70A/F108Y

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158651

    Purpose

    making transgenics expressing a UAS driven YFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Tags

    Expression

    Mutation

    F70A/F108Y

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-Puro-sgAMPKa2

    Plasmid

    #138685

    Purpose

    Expresses a human AMPKa2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgAMPKa2 human (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUW-hSNCA-NE

    Plasmid

    #215486

    Purpose

    Lentiviral expression of human alpha-synuclein in mammalian cells

    Depositor

    Ophir Shalem

    Article

    Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Lentiviral

    Tags

    NE tag

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Tet-pLKO-puro shKRAS

    Plasmid

    #116871

    Purpose

    Tet-inducible suppression of KRAS

    Depositor

    William Hahn

    Article

    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

    Insert

    shKRAS (KRAS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid

    #214230

    Purpose

    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

    Insert

    cSrc kinase SH2 domain (SRC Human)
    

    Use

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid

    #135011

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    126aa domain from HSV-1 UL12 fused to the N-termi…

    Expression

    Mammalian

    Mutation

    126aa domain from HSV-1 UL12 fused to the N-termi…

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti.PGK.blast-Flag-MFF-S155,172D

    Plasmid

    #74442

    Purpose

    Lentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Asp, Ser 172 Asp (Numbering based on MFF …

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pGL4.27_eSOX17

    Plasmid

    #195498

    Purpose

    Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the extended endoderm distal enhancer of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Full distal endoderm enhancer region of SOX17 (SOX17 Human)
    

    Use

    Tags

    minP_luc2-hPEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL4.27_eSOX17.2

    Plasmid

    #195499

    Purpose

    Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Core distal endoderm enhancer region of SOX17 (SOX17 Human)
    

    Use

    Tags

    minP_luc2-hPEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mHDAC7-Spliced (1-938) pEF6-V5 (MJS_014) [Edit]

    Plasmid

    #73267

    Purpose

    Expression of mouse HDAC7-S (1-938) in mammalian cells (V5 tag)

    Depositor

    Matthew Sweet

    Article

    Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

    Insert

    HDAC7 (Hdac7 Mouse)
    

    Use

    Tags

    His/V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge