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pLPC-mCh-ACTB-P2A-EGFP

Plasmid

#165158

Purpose

Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

Depositor

Taekjip Ha

Article

Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

Insert

mCherry-ACTB-P2A-EGFP (ACTB Human)

Use

Retroviral

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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EBFP2-Cx43

Plasmid

#69020

Purpose

Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Tags

EBFP2

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP G116A

Plasmid

#123081

Purpose

Gateway entry clone encoding human GABARAP G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway entry vector / entry clone

Mutation

Glycine 116 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-VWF

Plasmid

#124823

Purpose

Expresses multimeric human VWF protein without A1 domain. This construct has no tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain (VWF Human)

Expression

Mammalian

Mutation

Deleted A1 domain

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLXSN-FLK1 TM

Plasmid

#65249

Purpose

Expression of dominant negative FLK1 in mammalian cells

Depositor

Axel Ullrich

Article

Millauer et al Nature. 1994 Feb 10;367(6463):576-9.

Insert

FLK1 TM (Kdr Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

deleted AA 786-1346

Promoter

LTR

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
RnBarr1-mTurquoise2

Plasmid

#137789

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_C_ERBB2

Plasmid

#187776

Purpose

MAC-tagged gene expression of human ERBB2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ERBB2 (ERBB2 Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-MED6

Plasmid

#15411

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED6 (Med6 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pHH0103_PRKACA_C1+C2

Plasmid

#110027

Purpose

Protein expression and purification of PRKACA_C1+C2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PRKACA_C1+C2 (PRKACA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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EGFR∆550-580-mEGFP

Plasmid

#203788

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

EGFR (EGFR Human)

Tags

mEGFP

Expression

Mammalian

Mutation

aa 550-580 deleted

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Actin-7

Plasmid

#55399

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Actin (ACTB Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF

Plasmid

#17797

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment only, amino acids 676-1…

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
mCerulean3-Alpha-5-Integrin-12

Plasmid

#55401

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Alpha-5 Integrin (ITGA5 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pJW1241

Plasmid

#74904

Purpose

Expresses 3XFLAG::GR LBD::eGFP from the hsp16.48 promoter

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

3X FLAG::GR LBD::eGFP (NR3C1 Nematode, Synthetic, Human)

Expression

Worm

Promoter

hsp16.48

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA1-GATA4-opposite orientation

Plasmid

#51928

Purpose

for T7 directed antisense production of mouse GATA4

Depositor

Jeffery Molkentin

Article

Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

Insert

GATA4 reverse orientation (Gata4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only
-111 hTF m2

Plasmid

#15449

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

All 3 Sp1 sites mutated

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4 (E317K)

Plasmid

#29528

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed glutamic acid 317 to lysine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pETM41_KEAP1_KELCH

Plasmid

#178476

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KEAP1_KELCH (KEAP1 Synthetic, Human)

Tags

His-MBP

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pISH-Hopx

Plasmid

#74347

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Hopx. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

HOP homeobox (Hopx Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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miniSOG-Tubulin-6

Plasmid

#57777

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits