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pST_APOE_HUMAN_V153A_G131S_11s (pBS0850)

Plasmid

#185281

Purpose

For the mammalian expression of the human protein APOE_HUMAN_V153A_G131S_11s. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_V153A_G131S_11s

Expression

Mammalian

Mutation

V153A, G131S

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E2_255-317 (pBS0848)

Plasmid

#185280

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E2_255-317. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E2_255-317

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E2_47-300 (pBS0846)

Plasmid

#185278

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E2_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E2_47-300

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_VrDHN1a (or YSK2) (pBS0807)

Plasmid

#185266

Purpose

For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a (or YSK2). This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_VrDHN1a (or YSK2)

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_TaDHN5 (pBS0801)

Plasmid

#185262

Purpose

For the mammalian expression of the durm wheat protein DHN_TaDHN5. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_TaDHN5

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_OsLEA5 (pBS0793)

Plasmid

#185259

Purpose

For the mammalian expression of the rice plant protein DHN_OsLEA5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_OsLEA5

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AdHsAPO-44 (pBS0746)

Plasmid

#185237

Purpose

For the mammalian expression of the Chinese giant salamander and human fusion protein AdHsAPO-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AdHsAPO-44

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOA4_ANDDA_52-358 (pBS0744)

Plasmid

#185235

Purpose

For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_52-358. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOA4_ANDDA_52-358

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_095

Plasmid

#180524

Purpose

dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_324

Plasmid

#180566

Purpose

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-TVA-eGFP-N2cG

Plasmid

#175440

Purpose

Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA

Plasmid

#173213

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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S113

Plasmid

#174551

Purpose

Constitutive PhCMV-driven mammalian expression vector of CDH-3-TF cassette (PhCMV-Gal4-mdm2-P2A-LD6-Rel65-pA)

Depositor

Bruno Correia

Article

Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

Insert

mdm2 and designed protein LD6 (MDM2 )

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_A37I-A44L

Plasmid

#162583

Purpose

Expresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

A37I and A44L

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_A893E-talin1 (1-2541)

Plasmid

#166116

Purpose

For expression of full-length mouse talin-1 carrying mutation A893E in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

A893E-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

A893E

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_L1539P-talin1 (1-2541)

Plasmid

#166119

Purpose

For expression of full-length mouse talin-1 carrying mutation L1539P in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

L1539P-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

L1539P

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_L2509P-talin1 (1-2541)

Plasmid

#166123

Purpose

For expression of full-length mouse talin-1 carrying mutation L2509P in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

L2509P-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

L2509P

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_S1750F-talin1 (1-2541)

Plasmid

#166120

Purpose

For expression of full-length mouse talin-1 carrying mutation S1750F in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

S1750F-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

S1750F

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_D2086V-talin1 (1-2541)

Plasmid

#166122

Purpose

For expression of full-length mouse talin-1 carrying mutation D2086V in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

D2086V-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

D2086V

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_R1368W-talin1 (1-2541)

Plasmid

#166117

Purpose

For expression of full-length mouse talin-1 carrying mutation R1368W in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

R1368W-talin1-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

R1368W

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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