We narrowed to 10,667 results for: plasmids 101

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pDSG578

Plasmid

#214215

Purpose

integrase plasmid

Depositor

Uri Alon

Article

Glass et al Cell. 2024 Jan 31:S0092-8674(24)00061-8.

Insert

PhlF, A118

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pZNpF-deleted

Plasmid

#245372

Purpose

Negative control, luciferase cassette deleted

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSAV108

Plasmid

#226323

Purpose

Empty backbone plasmid for genomic insertions into H. neapolitanus neutral site

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-EFS-CasRx-pA

Plasmid

#192488

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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OA-986F (AAEL003877-dCas9-VPR)

Plasmid

#183993

Purpose

Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

AAEL003877-dCAS9-VPR

Use

CRISPR

Tags

dCas9-VPR

Expression

Insect

Promoter

AAEL003887

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-0.5Syn-CasRx-pA

Plasmid

#192492

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.5Syn

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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gh8

Plasmid

#106692

Purpose

expression of gRNA targeting GALNT8

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT8 (GALNT8 Human)

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pIG-829_HA-GD2-28z_CAR_JUN_Retroviral

Plasmid

#207506

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

JUN, HA-GD2-28z_CAR (JUN Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

Plasmid

#207507

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-828_HA-GD2-28z_CAR_BATF_Retroviral

Plasmid

#207505

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF, HA-GD2-28z_CAR (BATF Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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DRH002_scAAV-hSyn-delta_iCre-HA

Plasmid

#225087

Purpose

Expression of inactive delta-iCre (control) with an HA tag in neurons driven by human synapsin promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

delta_iCre

Use

AAV and Cre/Lox

Tags

HA

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 D614G

Plasmid

#164437

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#141210

Purpose

empty GFP vector for cloning sgRNA or pgRNAs

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLY088_AAV_EFS-BCMA-41BBz-PRODH2

Plasmid

#192191

Purpose

BCMA CAR AAV vector PRODH2 KI (pLY088)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

BCMA CAR AAV vector PRODH2 KI (pLY088) (TNFRSF17 Synthetic)

Use

AAV; Mammalian expression

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS D614G soluble Spike

Plasmid

#164651

Purpose

Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 soluble spike (S SARS-CoV-2)

Tags

HIS

Expression

Mammalian

Mutation

D614G

Promoter

CAG

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only
pGL3-noSV40-humanEC1.25_S3-4-5-6

Plasmid

#173956

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer regions 3-6 (S3, S4, S5 and S6) from SOX9 enhancer cluster EC1.25

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.4 (V3G,S658N)

Plasmid

#212504

Purpose

Encodes SARS-CoV-2 variant BA.4 Spike V3G,S658N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.4 (contains third aa mutation and without N658S) (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

Plasmid

#207493

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-851_HA-GD2-28z_HA-TFAP4

Plasmid

#207501

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-hfCas13d-HA-mCherry-pA

Plasmid

#233029

Purpose

To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d-mCherry

Use

AAV

Tags

mCherry

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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