We narrowed to 1,878 results for: FAST
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Plasmid#113898PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligaseDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6PromoterCMV-MIE-TetO2Available SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only
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cycLuc_TEVS
Plasmid#119207PurposeCircularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with TEVs cleavage site
UseLuciferaseTags6xHis and AU1 tagExpressionMammalianPromoterCMVAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-mVenus-Twin-Strep
Plasmid#113891PurposeMammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusionDepositorTypeEmpty backboneUseLentiviralTags3C-mVenus-Twin-StrepPromoterCMV-MIE-TetO2Available SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT-Clover-BlastR
Plasmid#138569PurposeUniversal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJDepositorInsertClover-BlastR
UseCRISPRAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10
Plasmid#162387PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
Tags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2
Plasmid#113886PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepPromoterCMV-MIE-TetO2Available SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PYL1_nTEVp
Plasmid#119213PurposeExpresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domainDepositorInsertsplit nTEVp in sufion with PYL1
TagsAU1 tagExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ASAP2s
Plasmid#101276PurposeGenetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1DepositorInsertASAP2s
UseAAVExpressionMammalianPromoterhSyn (human synapsin)Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABI_cTEVp
Plasmid#119214PurposeExpresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domainDepositorInsertsplit cTEVp in fusion with ABI
TagsMyc tagExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ M2rtTA_CAGG TetON-Sox10 with GFP
Plasmid#115241PurposeRMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668)DepositorAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetON-GFP
Plasmid#115495PurposeLentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)DepositorInserteGFP
UseLentiviralAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_RFP
Plasmid#136979PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker RFP
ExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…Available SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_fast_dead_mt
Plasmid#167272PurposeOverexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
TagsHis x10ExpressionBacterialMutationE30R-C145APromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZ M2rtTA_CAGG TetON-Sox10
Plasmid#115240PurposeRMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)DepositorInsertSOX10 (SOX10 Human)
ExpressionMammalianAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Venus
Plasmid#136980PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
ExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…Available SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE
Plasmid#225708PurposeCre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5DepositorHas ServiceAAV9InsertASAP5-Kv2.1
UseAAVPromoterEF1αAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Tq2CFP
Plasmid#136981PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
ExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…Available SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-2P
Plasmid#179458PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-2P
ExpressionMammalianPromoterCAGAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-iGluSnFR4f-NGR-WPRE
Plasmid#234438PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorHas ServiceAAV1InsertiGluSnFR4f-NGR
UseAAVTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.1
Plasmid#111053PurposeExpresses dLight1.1 in mammalian cellsDepositorInsertdLight1.1
TagsFlag tagExpressionMammalianPromoterCAGAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only