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pBS-SKII-3XHA-fCLIP-URA

Plasmid

#188922

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-URA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-his6-HA3-CLIP

Plasmid

#188942

Purpose

T7 expression vector for making his6- and HA3-tagged CLIP protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-CLIP

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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LexAop2-myr::4xCLIPf

Plasmid

#87636

Purpose

For creating drosophila transgenics expressing LexAop2-myr4x::CLIPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xCLIPf

Expression

Insect

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLJD vector

Plasmid

#34595

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pCS2+-Zeo

Plasmid

#221725

Purpose

Expression of Zeocin resistance marker in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fCLIP-GS

Plasmid

#188939

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-GS

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fSNAP-GS

Plasmid

#188938

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fSNAP-GS

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-URA

Plasmid

#188927

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-URA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Phleomycin

Plasmid

#188925

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Phleomycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Hygromycin

Plasmid

#188924

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Phleomycin

Plasmid

#188920

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Phleomycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Hygromycin

Plasmid

#188919

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJH805_pJH_LldR_Pcon-sfGFP.0TAG

Plasmid

#163736

Purpose

Constitutive expression of green fluorescent protein encoding zero stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pWDY1228

Plasmid

#138594

Purpose

Expresses NatL2-R156K in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Mutation

changed Arginine 156 to Lysine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pFGL1269R

Plasmid

#118994

Purpose

Marker fusion of Bar:mCherry (without start codon)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

Bar:mCherry

Use

Fungal expression

Promoter

promoter less

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1259

Plasmid

#118996

Purpose

GFP (without start and stop codons) in pFGL1010

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

eGFP

Use

Fungal expression

Promoter

promoter less

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 dATG1 (1188)

Plasmid

#66918

Purpose

bacterial expression of dATG1

Depositor

Michael Karin, Jun Hee Lee

Article

Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

Insert

ATG1

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pSCT-GAL93-DEST

Plasmid

#46339

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Type

Empty backbone

Tags

6XHIS

Expression

Mammalian

Promoter

pCMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
tout velou (ttv)

Plasmid

#37401

Depositor

Norbert Perrimon

Article

Bellaiche et al Nature. 1998 Jul 2;394(6688):85-8.

Insert

tout-velu (ttv Fly)

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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