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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,499 results for: multiplex

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Showing: 321 - 340 of 3499 results

  4. pYPQ151

    Plasmid
    #69302
    Purpose
    Gateway entry vector with pcoCas9D10A
    Depositor
    Yiping Qi
    Publication
    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pcoCas9D10A (Plant codon-optimized)
    Use
    CRISPR
    Tags
    2XFLAG
    Mutation
    D10A mutation; NLS fusion
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69302-plasmid-map-sequence-id-122140

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  7. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid
    #48227
    Purpose
    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    Use
    CRISPR
    Tags
    2A-neo, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48227-plasmid-map-sequence-id-69517

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  8. pBUN6I11

    Plasmid
    #50580
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50580-plasmid-map-sequence-id-75637

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  16. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid
    #104571
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    Use
    CRISPR
    Tags
    NLS-3xHA-3xFLAG-4xDmrA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104571-plasmid-map-sequence-id-201436

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  18. pG3H-PE3max-attR1R2

    Plasmid
    #213049
    Purpose
    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites
    Depositor
    Bing Yang
    Publication
    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1
    Inserts
    Cas9 nickase
    M-MLV Reverse Transcriptase
    Use
    Note: this plasmid needs helper pvs1-vir2 for rep…
    Expression
    Plant
    Mutation
    H840A, R240K, N413K,
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213049-plasmid-map-sequence-id-422145

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Showing: 321 - 340 of 3499 results