We narrowed to 6,987 results for: p2a

1. pHR_TRE_Ncad-P2A-GFPlig

   Plasmid

   #133806

   Purpose

   inducible expression of Ncad and membrane-tethered GFP in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   cadherin 2 (CDH2) (Cdh2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

   Plasmid

   #170209

   Purpose

   Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus

   Depositor

   Kelly Monk

   Article

   Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

   Insert

   slc1a3b (slc1a3b Zebrafish)
   

   Use

   Zebrafish

   Tags

   myrGCaMP6s-P2A-H2AmCherry

   Expression

   Mutation

   Promoter

   slc1a3b

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

   Plasmid

   #52421

   Purpose

   Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

   Depositor

   Bertrand Collet

   Insert

   Fusion NLS-EGFP-P2A-Cherry-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2attNG-H11-short

   Plasmid

   #51546

   Purpose

   p2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).

   Depositor

   Michele Calos

   Article

   Zhu et al Nucleic Acids Res. 2013 Dec 4.

   Insert

   GFP4m
   

   Use

   Human targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_TRE_mcherry-P2A-Pcad

   Plasmid

   #133807

   Purpose

   inducible expression of mCherry and Pcad in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   cadherin 3 (CDH3)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

   Plasmid

   #82024

   Purpose

   Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   mKate2-2xNLS
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cre-p2a-tdTomato

   Plasmid

   #176837

   Purpose

   Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Cre-P2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

   Plasmid

   #196677

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   PKIα-P2A-mRuby3
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-RAP2A-GFP

   Plasmid

   #86124

   Purpose

   Retroviral vector expressing RAP2A-GFP-IRES-Bsd

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Retroviral

   Tags

   3xFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-SRGAP2A-EGFP

    Plasmid

    #168501

    Purpose

    Expression of full length SRGAP2 with a C-termonal EGFP fusion

    Depositor

    Franck Polleux

    Article

    Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

    Insert

    SRGAP2A (SRGAP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5 HA GST rap2a

    Plasmid

    #14952

    Depositor

    David Sabatini

    Article

    Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

    Insert

    rap2a (RAP2A Human)
    

    Use

    Tags

    HA-GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

12. SPLICS Po-ER Short P2A

    Plasmid

    #164114

    Purpose

    Detect the long Peroxisomes-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP Po-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2attNG-H11-long

    Plasmid

    #51545

    Purpose

    p2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.

    Depositor

    Michele Calos

    Article

    Zhu et al Nucleic Acids Res. 2013 Dec 4.

    Insert

    GFP4m
    

    Use

    Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AICSDP-41: ATP2A2-mEGFP

    Plasmid

    #114405

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2

    Depositor

    Allen Institute for Cell Science

    Insert

    ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBT2-4xUAS:P2A-mCerulean3

    Plasmid

    #127592

    Purpose

    This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

    Depositor

    Longhou Fang

    Article

    Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2attB-FST344-HA-CPL

    Plasmid

    #131460

    Purpose

    Encodes human Follistatin protein

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    Follistatin (FST Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCas9-P2A-BFP

    Plasmid

    #149447

    Purpose

    Expresses Cas9 and BFP in a lentiviral vector.

    Depositor

    Jonathan Chernoff

    Article

    Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

    Insert

    P2A-BFP
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti_Ef1a:PE2-P2A-hMLH1dn

    Plasmid

    #184444

    Purpose

    Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Ef1A

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMIG-PP2A-Abeta

    Plasmid

    #15248

    Depositor

    William Hahn

    Article

    Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

    Insert

    PP2A regulatory subunit A, beta isoform (PPP2R1B Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pLNCX2-mEGFP-CHMP2A

    Plasmid

    #115328

    Purpose

    expresses mEGFP-CHMP2A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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