Purpose
A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFĪHTH::Ī»N22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
Inserts
dcas9
tetR
pspFĪHTH::Ī»N22plus
sfgfp
Tags
ASV tag and pspFĪHTH::Ī»N22plus
Mutation
Mutations D10A and H840A on WT cas9. Synonymous mā¦
Promoter
Anderson promoter: J23106, PpspA-LEA3B3, and Ptet
Available since
Sept. 20, 2019
Availability
Academic Institutions and Nonprofits only