We narrowed to 5,949 results for: PEP
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Plasmid#218915PurposePiggyBac transpositionDepositorInsertV5-TurboID-MUTT2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
ExpressionMammalianMutation2363G>C and 4330T>CAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA
Plasmid#218913PurposePiggyBac transpositionDepositorInsertV5-TurboID-MUTT2A-SARS-CoV2-NCAP/BlastR (N )
ExpressionMammalianMutation2363G>C and 5335T>CAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA
Plasmid#218912PurposePiggyBac transpositionDepositorAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
GRPR-DuET
Plasmid#213306PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FPR2-DuET
Plasmid#213240PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_CRF0.5
Plasmid#208660PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
UseAAVPromoterhSynAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)
Plasmid#155134PurposeExpresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
TagsHisExpressionBacterialMutationC104A/D243G/L244G/F245G/M247C/S248G/M249G/A250G, …PromoterT7Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3e_NAB
Plasmid#156473PurposeBacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tagDepositorInsertNsp3e_NAB (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v5
Plasmid#145152PurposeMammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 5DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-T92Q
Plasmid#145156PurposeMammalian expression plasmid for soluble ACE2 (protease domain) T92Q glycosylation mutantDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shCrh2
Plasmid#132710PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh geneDepositorAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]
Plasmid#106469PurposeExpresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(S219,D415,H474A)
TagsGFPExpressionBacterial and YeastMutationChanged Serine 219, Aspartate 415 and Histidine 4…PromoterYBR139WAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS-FCER1G-1
Plasmid#109192PurposeEncodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression systemDepositorAvailable SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC293 - pAAV EF1a V5-synuclein (WT)
Plasmid#60057PurposeAn AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.DepositorAvailable SinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-WT(732)-IgG1
Plasmid#154104PurposeMammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable SinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGHUC w-ERBIN (2-hybrid prey plasmid)
Plasmid#128163PurposeUV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).DepositorInsertERBIN PDZ domain (ERBIN Synthetic)
UseSynthetic BiologyTagsFLAG and Omega subunit of RNA polymeraseExpressionBacterialPromoterUV5Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)-sfGFP
Plasmid#145171PurposeMammalian expression plasmid for soluble ACE2-super folder GFP fusionDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV hPGRN
Plasmid#213682PurposeExpresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tagDepositorInsertHuman Progranulin (hPGRN) (GRN Human)
UseAAVTagsTwin-Strep tag and V5 tag (after signal peptide)Promotercytomegalovirus enhancer/chicken β-actin promoterAvailable SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-WT(732)-8h
Plasmid#154101PurposeMammalian expression plasmid for dimeric soluble ACE2 (8his tag)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable SinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRM053 His-TEV-Cas9 triNLS 0C
Plasmid#196244PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only