We narrowed to 12,340 results for: pcDNA3

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pcDNA3.1-Y1214F-VEGFR2-mCherry

Plasmid

#139609

Purpose

Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ClopHensor-H148G-V224L

Plasmid

#46366

Depositor

Daniele Arosio

Article

Mukhtarov et al Front Mol Neurosci. 2013 Apr 18;6:9. doi

Insert

ClopHensor-H148G-V224L

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP-H148G-V224L is EGFP-T203Y-H148G-V224L

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 Mito-Ras-LOCKR-S

Plasmid

#216501

Purpose

FRET-based reporter for Ras activity, mitochondria localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Mito-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PhLP1-myc-6xHis

Plasmid

#218176

Purpose

Expresses phosducin-like protein 1 in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

Phosducin like protein 1 (PDCL Human)

Tags

6x His and Myc

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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Rab8-T22N(Flag) in pcDNA3.1neo

Plasmid

#46784

Purpose

expression of T22N Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A T22N (RAB8A Human)

Tags

FLAG

Expression

Mammalian

Mutation

T22N dominant negative

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/TO-myc-BirA*-NES

Plasmid

#112714

Purpose

For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

HIV Rev nuclear export signal (NES)

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR-NES

Plasmid

#118407

Purpose

Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR-NES

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-G97 IRES EGFP

Plasmid

#130711

Purpose

Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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RelA RHD cFlag pcDNA3

Plasmid

#20015

Depositor

Stephen Smale

Insert

RelA (Rela Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Contains only the Rel Homology Domain of RelA. It…

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-Akt1-aa1-408

Plasmid

#73412

Purpose

Expresses HA tagged aa1-408 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-408 of AKT1

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-Akt-STOPS

Plasmid

#175009

Purpose

Plasma membrane-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

Lyn-mCherry-Akt-STOPS

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-205

Plasmid

#26312

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-205 (MIR205 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mCherry-CyrCR1.miR16

Plasmid

#128747

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(P29S)

Plasmid

#224280

Purpose

Cell transfection and expression of Rac1 (P29S)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

P29S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (kinase dead)

Plasmid

#29533

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 6A CARD8 CARD-FLAG WT

Plasmid

#164020

Purpose

Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMJA219= pcDNA3.1V5-completeTRABV-CMVp

Plasmid

#116878

Purpose

mammalian expression vector containing TCR sequences

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

complete TRAV10-TRBV25-CMVp rabbitpolyA (TRAV10, TRBV25-1 Human)

Expression

Mammalian

Promoter

CMV Promoter

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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1553 pcDNA3 HA gamma adaptin 2

Plasmid

#10713

Depositor

William Sellers

Insert

Gamma adaptin 2 (AP1G2 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
GeNL(Ca2+)_60/pcDNA3

Plasmid

#85207

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_60

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-W340A.pcDNA3-k

Plasmid

#127404

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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