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pSpCas9_T2A_mCherry sgBFP

Plasmid

#192929

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry Codon 59 sgRNA

Plasmid

#109431

Purpose

MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

mCherry codon 59 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hRad51(S208E-A209D)–Cas9(D10A)

Plasmid

#125561

Purpose

Expresses the hRad51(S208E-A209D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(S208E A209D)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE Flag HA ADAR2

Plasmid

#237856

Purpose

Lentiviral expression of ADAR2

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

ADAR2 (ADARB1 Human)

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLBPVXBa-M

Plasmid

#229079

Purpose

New version of a PVX vector to express heterologous sequences in plants

Depositor

Jose-Antonio Daros

Article

Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

Insert

Potato virus X

Expression

Plant

Mutation

Mutation coat protein start codon (ATG to AGG); d…

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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hEGFR-mEGFP

Plasmid

#182878

Purpose

Mammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFP

Depositor

Salvatore Chiantia

Article

Tzoneva et al Biomolecules. 2020 May 22;10(5). pii: bi

Insert

hEGFR-mEGFP (EGFR Human)

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-CDDR

Plasmid

#149355

Purpose

CRISPR/Cas9-based dual-fluorescent DSB repair reporter

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

CDDR Reporter
Blasticidin resistance

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a and hPGK

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 0819

Plasmid

#118704

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLIX401 C-term SETX

Plasmid

#237999

Purpose

Inducible lentiviral expression of C-term SETX

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

SETX (SETX Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pC3.1_CMV_oROS-G

Plasmid

#216111

Purpose

Expresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv [Preprint]. 2024 Feb 4:2024.01.3

Insert

oROS-G

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-V5-KDM3A

Plasmid

#82331

Purpose

lentiviral expression of KDM3A

Depositor

Luke Gaughan

Article

Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-20

Insert

KDM3A (KDM3A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

two silent mutations to make it siRNA resistant

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only
Cas9-sgRNA-A

Plasmid

#149369

Purpose

Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

Cas9
sgRNA-A
Puromycin resistance

Use

CRISPR

Tags

3xFLAG, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CBh, PGK, and U6

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDule-pCNF

Plasmid

#85494

Purpose

Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

Insert

para-cyanophenylalanine Mj synthetase

Tags

None

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pIRES_EGFP_GA_KCNE1

Plasmid

#173160

Purpose

For mammalian expression of KCNE1 and creating variants

Depositor

Al George

Article

Kuenze et al Elife. 2020 Oct 23;9. pii: 57680. doi: 1

Insert

KCNE1 (KCNE1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEND-321_Cacnes_ori

Plasmid

#225603

Purpose

Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti Dest BlastR R1-R2

Plasmid

#84574

Purpose

Gateway destination vector expressing Blasticidin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-trGluc

Plasmid

#158959

Purpose

DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

trGluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLAMP1-miRFP703

Plasmid

#79998

Purpose

LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

LAMP1 (Lamp1 Rat)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDRM98 mKate2 Blast

Plasmid

#174720

Purpose

Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

mKate2
BSD

Use

Lentiviral

Tags

P2A linker and SV40 NLS with GGS linker

Expression

Mammalian

Promoter

MND and MND (off mKate-P2A)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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