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GB1-His-TEV-A7 (2-180)

Plasmid

#190066

Purpose

Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 PRD (ANXA7 Human)

Tags

GB1-His-TEV

Expression

Bacterial

Mutation

PRD only, residues 2-180

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-His-TEV-A7-Strep (2-180)

Plasmid

#190067

Purpose

Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 PRD (ANXA7 Human)

Tags

GB1-His-TEV and Strep

Expression

Bacterial

Mutation

PRD only, residues 2-180

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BBLF2/3

Plasmid

#195831

Purpose

Gateway Entry Clone of Epstein-Barr virus BBLF2/3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BBLF2/3 (BBLF2/BBLF3 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BcRF1(vTBP)

Plasmid

#195832

Purpose

Gateway Entry Clone of Epstein-Barr virus BcRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BcRF1 (BcRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BCRF1(vIL10)

Plasmid

#195777

Purpose

Gateway Entry Clone of Epstein-Barr virus BCRF1, vIL-10

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BCRF1, vIL-10 (BCRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BaRF1(RNR-s)

Plasmid

#195795

Purpose

Gateway Entry Clone of Epstein-Barr virus BaRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BaRF1 (BaRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ230-D156R

Plasmid

#188542

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-D156R

Use

CRISPR; Gateway compatible lbcas12a-d156r entry c…

Tags

NLS

Expression

Plant

Mutation

D156R

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-BCAS2

Plasmid

#192294

Purpose

Gateway entry vector for an inducible 3xFLAG-BCAS2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BCAS2 (BCAS2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-DYNLL2

Plasmid

#192296

Purpose

Gateway entry vector for an inducible 3xFLAG-DYNLL2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

DYNLL2 (DYNLL2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pOPO794

Plasmid

#195303

Purpose

pBAD322-MycaIDCas-ΔCas6-RGR-PaqCI, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked PaqCI entry site.

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

McCAST Cascade Δcas6 and ribozyme flanked entry site

Expression

Bacterial

Promoter

Arabinose inducible arapBAD

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-DrCavin1b

Plasmid

#194292

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJ

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK2-mC-S

Plasmid

#172428

Purpose

Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK2 (KCNJ6 Human)

Tags

mCherry Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C G83R

Plasmid

#184747

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, G83R

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UIM-mut

Plasmid

#187147

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

A1376G/S1382A

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB_EA_3EGFP

Plasmid

#185967

Purpose

Expresses EGFP from PR promoter in E.coli Dh5alpha cell

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Pr-Egfp cassette in forward direction in Network Brick

Use

Synthetic Biology

Promoter

PR

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C Y78F

Plasmid

#184746

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, Y78F

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C A81T

Plasmid

#184745

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, A81T

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C K80E

Plasmid

#184744

Purpose

bacterial expression of huamn TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, K80E

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA/UIM-mut

Plasmid

#187151

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

M1328A/F1330A/A1376G/S1382A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBM-mut

Plasmid

#187149

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

L2976A/P2977A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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