We narrowed to 89,966 results for: Mal;

1. pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199364

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LbCas12a-DR-cr in PBCSK-

   Plasmid

   #126641

   Purpose

   Cloning vector LbCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 Promoter

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. All-in-one split-ADAR2

   Plasmid

   #170136

   Purpose

   AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-mCherry_SNAP_SNAPV_PACT_Dead

   Plasmid

   #173073

   Purpose

   Dead SNAP tag at the centrosome

   Depositor

   Heidi Hehnly

   Article

   Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

   Insert

   mCherry-SNAP-SNAP-Varied-PACT
   

   Tags

   mCherry-SNAP-SNAP-Varied

   Expression

   Mammalian

   Mutation

   AA144 in both SNAP tags changed from a cysteine t…

   Promoter

   CMV IE94

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJZC42

   Plasmid

   #66564

   Purpose

   sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 1XPP7
   PCP-VP64 IRES mCherry
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HCMVgH (Merlin)

   Plasmid

   #136448

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GluR2_adRNA(1,20,6)-ADAR2

   Plasmid

   #124620

   Purpose

   Expresses adRNA (1,20,6) targeting the RAB7A transcript along with the ADAR2

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   ADAR2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SMVP-Cas9N-U6-gRNA TdL

   Plasmid

   #80938

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNAIIIB rhoA WT

   Plasmid

   #61631

   Purpose

   mammalian expression of rhoA

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   rhoA (RHOA Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

10. dCas9-NLS-gs10-cGR2176

    Plasmid

    #188258

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cGR2176
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUp95GW (B7)

    Plasmid

    #74016

    Purpose

    lentiviral expression of shRNA resistant Psd95-EGFP fusion

    Depositor

    Robert Malenka, Oliver Schluter, Weifeng Xu

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 (DLG4 Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. GWF180

    Plasmid

    #133607

    Purpose

    CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    DNCR2-VPR, NS3aH1-tagBFP-SpdCas9
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-Flag-hDcp2

    Plasmid

    #72213

    Purpose

    mammalian expression of Flag-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q170R (please see depositor's comment below)

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQsupR-Mi2b

    Plasmid

    #15379

    Depositor

    Stephen Smale

    Article

    Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

    Insert

    short hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)
    

    Use

    RNAi and Retroviral

    Available Since

    April 18, 2008

    Availability

    Academic Institutions and Nonprofits only

15. GFP-Myo 1e

    Plasmid

    #134905

    Purpose

    Expression of rat Myo 1e (Myr 3) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Stoffler et al J Cell Biol. 1995 May;129(3):819-30. doi

    Insert

    Myo 1e (rat) with 3' UTR (Myo1e Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. OVA-miSFIT-8-G

    Plasmid

    #124675

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. BoxB-MS2 adRNA (RAB7A-ACG site)

    Plasmid

    #170155

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(ACG)-MS2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX-FKBP-BclXL-Blasticidin

    Plasmid

    #120718

    Purpose

    Expresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Bl2-like protein 1, isoform Bcl-X(L)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    33 c-terminal amino acids

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEntry Tshld(FKBP F36V)-GFP

    Plasmid

    #53260

    Purpose

    pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.

    Depositor

    Matthew Pratt

    Article

    Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

    Insert

    Tshld(FKBP F36V)-GFP
    

    Tags

    EGFP, Flag, and HA

    Mutation

    FKBP F36V

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDN-D2irTN2AG5kwh

    Plasmid

    #128253

    Purpose

    Mammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    Beta Globin Intron::hTetR::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    CMV D2ir promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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