We narrowed to 4,343 results for: TIL

1. pTRE-BI-TRIM21

   Plasmid

   #207838

   Purpose

   Inducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   TRIM21 (TRIM21 Human)
   mCherry
   hIgG1-FC
   VHH-GFP4
   

   Use

   Tags

   weak NLS

   Expression

   Mammalian

   Mutation

   All K mutated to R

   Promoter

   TRE3G BI promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS6-ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB

   Plasmid

   #111504

   Purpose

   Expresses a zinc finger specific to the human VEGFA locus, which is linked to a KRAB transcriptional repression domain via a StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

   Depositor

   Michael Lin

   Article

   Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

   Insert

   ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The HCV NS3 protease carries F43L, Q80K, S122R, a…

   Promoter

   CMV

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. GDNF-SHC

   Plasmid

   #139624

   Purpose

   StrepIII-HA-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   GDNF (GDNF Human)
   

   Use

   Tags

   StrepIII-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-TEV-mClover3

   Plasmid

   #105795

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV[Exp]-Puro-EF-1α>{H2B-RFP}

   Plasmid

   #201121

   Purpose

   RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

   Depositor

   Ori Bar-Nur

   Article

   Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

   Insert

   H2B-RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6_sgRNA_CAG_hSt1Cas9_LMD9

   Plasmid

   #110626

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and hU6

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. px330 Rosa26 Gu1 (LH416)

   Plasmid

   #99629

   Purpose

   Plasmid used to target the wildtype Rosa26 allele with Cas9

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-VTKS6

   Plasmid

   #170857

   Purpose

   AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MCP-3x9aaTAD

   Plasmid

   #210707

   Purpose

   This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-CARMA2sh-HA

    Plasmid

    #97072

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-VTKS5-GFP

    Plasmid

    #178713

    Purpose

    AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-VTKD4-dTom

    Plasmid

    #178715

    Purpose

    AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    dTom
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL5.0 hCortKD-Flcortmut3YD EGFP

    Plasmid

    #187264

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    3YD (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. hA3A-BE3 (pRZ215)

    Plasmid

    #131314

    Purpose

    CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX304-MARK3_T211A

    Plasmid

    #107236

    Purpose

    Lentiviral gene expression vector for phospho-MARK3_T221A expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MARK3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKK-mEGFP-TEV

    Plasmid

    #105777

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mEGFP-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-hSyn-SpdCas9-W3SL

    Plasmid

    #210708

    Purpose

    This Plasmid express hSyn promoter driven SpdCas9

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    S. Pyogenes dCas9
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    hSyn

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-CARMA2sh-FLAG

    Plasmid

    #97071

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-VTKS6-GFP

    Plasmid

    #178714

    Purpose

    AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLL5.0 mCherry-hCortKD-Flcortmut3YD

    Plasmid

    #187266

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    3YD (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge