We narrowed to 3,535 results for: mpo

1. PEA1-GFP

   Plasmid

   #171993

   Purpose

   Delivers all prime editing nickase components in a single, GFP selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-GGamma13-GFP2

   Plasmid

   #140992

   Purpose

   Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma13-GFP2 (GNG13 Human)
   

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-GGamma2-GFP2

   Plasmid

   #166774

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma2-GFP2 (GNG2 Synthetic, Human)
   

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-GGamma12-GFP2

   Plasmid

   #166781

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma12-GFP2 (GNG12 Synthetic, Human)
   

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-GGamma3-GFP2

   Plasmid

   #166775

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma3-GFP2 (GNG3 Synthetic, Human)
   

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-GGamma7-GFP2

   Plasmid

   #166778

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma7-GFP2 (GNG7 Synthetic, Human)
   

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-GGamma11-GFP2

   Plasmid

   #166780

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma11-GFP2 (GNG11 Synthetic, Human)
   

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV5-Flag-Ago2

   Plasmid

   #72207

   Purpose

   Mammalian expression of full-length human Ago2

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   argonaute RISC catalytic component 2 (AGO2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

   Plasmid

   #179843

   Purpose

   Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Luciferase and Synthetic Biology

   Tags

   eGFP and luciferase

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMX-hPGK-CD3-P2A-CD90.2

    Plasmid

    #163334

    Purpose

    Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the hPGK promoter

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD3
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR221 UBR5 ∆PABC

    Plasmid

    #81064

    Purpose

    Gateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)
    

    Use

    Cloning

    Mutation

    Y2402A and K2415A (bp t7204g, a7205c, t7206c, a72…

    Promoter

    N/A

    Available Since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB63 - pL0_fLUC-I (CDS1)

    Plasmid

    #123180

    Purpose

    Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
    

    Use

    Luciferase; Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMY-CD3-P2A-CD90.2

    Plasmid

    #163338

    Purpose

    Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the LTR promoter

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD3
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMX-CAG-CD3-P2A-CD90.2

    Plasmid

    #163336

    Purpose

    Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the CAG promoter

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD3
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121842

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven xCas9(3.7) gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA fusion to dCas9 w/ gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNAM-human eIF2C1-B-Myc

    Plasmid

    #50362

    Purpose

    Expression of human eIF2C1 mutant in mamalian cells

    Depositor

    Kumiko Ui-Tei

    Article

    Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

    Insert

    eIF2C1 (AGO1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contain PIWI domain

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNAM-human eIF2C1-A-Myc

    Plasmid

    #50361

    Purpose

    Expression of human eIF2C1 mutant in mamalian cells

    Depositor

    Kumiko Ui-Tei

    Article

    Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

    Insert

    eIF2C1 (AGO1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contains PRP motif and PAZ domains

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pBD-PYR1

    Plasmid

    #241278

    Purpose

    Yeast two hybrid vector expressing BD-PYR1 (wild type)

    Depositor

    Sean Cutler

    Article

    Park et al Science. 2009 May 22;324(5930):1068-71.

    Insert

    PYR1 (PYR1 Mustard Weed)
    

    Expression

    Yeast

    Mutation

    NA

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid

    #234370

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Inserts

    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    gRNA 1-1 and gRNA 1-2
    

    Use

    Synthetic Biology

    Tags

    GB1, GP41, and sfGFP

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid

    #234372

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Inserts

    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-24XGP41
    gRNA 1-1 and gRNA 1-2
    

    Use

    Synthetic Biology

    Tags

    GB1, GP41, and sfGFP

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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