We narrowed to 7,394 results for: 11

1. Cx26-sfGFP

   Plasmid

   #69026

   Purpose

   Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx26 (Gjb2 Rat)
   

   Tags

   non-monomerized superfolder GFP (V206)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

   Plasmid

   #140204

   Purpose

   Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Step 1 of cloning c…

   Promoter

   Aspergillus fumigatus U3 (RNAPIII).

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK6_016

   Plasmid

   #124058

   Purpose

   Encodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   WPRE (extended)
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATF4 15: 5’ATF4.uORF1stopm1.GFP

   Plasmid

   #21862

   Depositor

   David Ron

   Article

   Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

   Insert

   ATF4 (Atf4 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Stop codon for uORF1 converted to UCG (see author…

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

   Plasmid

   #65662

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   introduced HindIII site (final goal to exchange F…

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSuper.Retro.Puro-PKN1 shRNA 4

   Plasmid

   #59978

   Purpose

   Knockdown of PKN1

   Depositor

   Debu Chakravarti

   Article

   Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

   Insert

   PKN1 shRNA
   

   Use

   Retroviral

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pG418R-MCS-QF-MiniWhite

   Plasmid

   #165902

   Purpose

   G418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable Empty Enhancer Driver
   

   Use

   Synthetic Biology

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Venus-G protein Gamma 5

   Plasmid

   #42200

   Depositor

   Narasimhan Gautam

   Article

   Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

   Insert

   Gamma 5 (Gng5 Rat)
   

   Tags

   Venus(1-155)

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   CMV

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

9. MTK2_024

   Plasmid

   #123716

   Purpose

   Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pPGK1c
   

   Expression

   Mammalian

   Mutation

   Crippled Kozac sequence

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. M72-IRES-tauRFP2-ACNF TV

    Plasmid

    #15512

    Depositor

    Peter Mombaerts

    Article

    Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

    Insert

    M72-IRES-tauRFP2-ACNF TV (Olfr160 Mouse)
    

    Use

    Mouse Targeting

    Mutation

    Targeting vector to insert IRES-tauRFP2-ACNF imme…

    Available Since

    Aug. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

11. (E6) CMV-p18 C/EBPalpha

    Plasmid

    #25881

    Depositor

    Ormond MacDougald

    Article

    Erickson et al J Biol Chem. 2001 May 11. 276(19):16348-

    Insert

    p18 C/EBPalpha (Cebpa Mouse)
    

    Expression

    Mammalian

    Mutation

    N-terminal truncation, contains only CR4 and bZIP…

    Available Since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pWay21-MKP-1L16A/L17A

    Plasmid

    #13478

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1L16A/L17A (Dusp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Leucine 16 to Alanine; changed Leucine 1…

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

13. hSTARR-seq_OCT4 3UTR vector

    Plasmid

    #99295

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTW324

    Plasmid

    #115955

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P1 End
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTW064

    Plasmid

    #115951

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 ORF Dest
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. hSTARR-seq_mCP vector

    Plasmid

    #99291

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK3b_020

    Plasmid

    #123801

    Purpose

    Encodes synTF ZF binding domain as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    SynTF
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUPERpuro-BglII-Hs4E-T_AC

    Plasmid

    #148445

    Purpose

    Mammalian Expression of Hs4E-T-shRNA

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    Hs4E-T-shRNA (EIF4ENIF1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB3043(gRNA XI-1)

    Plasmid

    #73285

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Xl-VSP1 R152Q GFP

    Plasmid

    #51884

    Purpose

    expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 R152Q (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R152Q: near the extracellular side of the fourth …

    Promoter

    CMV

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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