We narrowed to 34,882 results for: CaS;

1. EF1a-dCas9-KRAB-GFP backbone

   Plasmid

   #194281

   Purpose

   Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-dCas9-ZIM3-KRAB-BFP

   Plasmid

   #196712

   Purpose

   CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-KRAB(ZIM3)-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. hRad51(S208E-A209D)–Cas9(D10A)

   Plasmid

   #125561

   Purpose

   Expresses the hRad51(S208E-A209D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51(S208E A209D)–Cas9(D10A) (RAD51 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRm-NLS-dCas9-GFP11x7-NLS

   Plasmid

   #70224

   Purpose

   Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   NLS-dCas9-GFP11x7-NLS
   

   Use

   Tags

   GFP11x7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

   Plasmid

   #167295

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAK (BAK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

   Plasmid

   #167296

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAX (BAX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AsCas12a-6xNLS-E174R/S542R (pRG232)

   Plasmid

   #149723

   Purpose

   Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

   Depositor

   Junwei Shi

   Article

   Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV hSyn-fDIO-taCasp3-TEVp

   Plasmid

   #207997

   Purpose

   Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase

   Depositor

   Kazunari Miyamichi

   Article

   Goto et al Neuron. 2025 Feb 17:S0896-6273(25)00041-

   Insert

   taCasp3-TEVp
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-TRE3G-dCas9-EGFP-HaloTag

    Plasmid

    #169451

    Purpose

    expression of dCas9-EGFP-HaloTag

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9-EGFP-HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_TRE3G-LSD1-dCas9-P2A-mCherry

    Plasmid

    #138462

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    LSD1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRE-KRAB-dCas9-IRES-BFP

    Plasmid

    #85449

    Purpose

    Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    KRAB-dCas9-IRES-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA Tag, and KRAB domain

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

    Plasmid

    #194284

    Purpose

    EF1a-dCas9-KRAB-GFP with nontarget sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid

    #139994

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

    Plasmid

    #159904

    Purpose

    Mutagenesis of Kcnn3

    Depositor

    Larry Zweifel

    Insert

    Kcnn3 (Kcnn3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. XLone-Puro Cas13d-eGFP U6 BbsI

    Plasmid

    #155184

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Type

    Empty backbone

    Use

    Unspecified; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC018 - LshCas13a from Leptotrichia shahii

    Plasmid

    #91908

    Purpose

    Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LshCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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