We narrowed to 13,613 results for: crispr cas9

1. pAf-CRISPR-yA

   Plasmid

   #191015

   Purpose

   CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

   Insert

   yA
   

   Use

   CRISPR

   Promoter

   Aspergillus flavus U6

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNOC-CRISPR-HA

   Plasmid

   #99370

   Purpose

   Expresses Cas9-HA and sgRNA. Hygromycin resistance.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-HA

   Promoter

   Ribi promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Cas9-T2A-TdT

   Plasmid

   #126424

   Purpose

   Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-TdT (DNTT Streptococcus pyogenes, Human)
   

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. px335-sgRNA-EF(Cas9n)-mHdac1-L2

   Plasmid

   #122307

   Purpose

   Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Hdac1 (Hdac1 Synthetic)
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHelper-CRISPRi-sgRNA

   Plasmid

   #221135

   Purpose

   Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   sgRNA-scaffold with dCas9 handle
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119(SpeI)

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

   Plasmid

   #164471

   Purpose

   Expresses sgRNA targeting human GJA1 locus

   Depositor

   Markus Kuehn

   Article

   Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

   Insert

   Gap junction alpha-1 (GJA1) (GJA1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9

   Plasmid

   #87917

   Purpose

   Gateway cloning

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   sgHTT1, sgGFP2, shCas9
   

   Expression

   Mammalian

   Promoter

   H1, U6, 7SK

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. L-CRISPR-CTN (MLL-ENL)

   Plasmid

   #69212

   Purpose

   Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

   Plasmid

   #233373

   Purpose

   EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Inserts

   NES-Kinprola_on-mEGFP
   FLAG-SV40NLS-Cas9-NLS-T2A-BSD
   

   Use

   Lentiviral

   Tags

   FLAG, NES, and mEGFP

   Promoter

   EF1α

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid

    #233372

    Purpose

    EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Inserts

    NES-Kinprola_PKA_T/A-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    

    Use

    Lentiviral

    Tags

    FLAG, NES, and mEGFP

    Promoter

    EF1α

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid

    #201916

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Expression

    Mammalian

    Promoter

    CAG and Human U6

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLR5-CBh-dCas9-hUtx-IRES-Hyg

    Plasmid

    #122374

    Purpose

    Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    CBh-dCas9-hUtx-IRES-Hyg-pA
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLR5-CBh-dCas9-hEzh2-IRES-Hyg

    Plasmid

    #122375

    Purpose

    Expresses dCas9-hEzh2-IRES-Hyg in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    CBh-dCas9-hEzh2-IRES-Hyg-pA
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS

    Plasmid

    #121834

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-Cas9(3.7) (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

    Plasmid

    #121828

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7) (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold

    Plasmid

    #121812

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

    Plasmid

    #246438

    Purpose

    All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.

    Depositor

    Ailong Ke

    Article

    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

    Inserts

    NmeCas9
    Nme-sgRNA
    

    Tags

    HA tag, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    changed Aspartic acid 16 to Alanine, deleted amin…

    Promoter

    EF-1 alpha and U6

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

    Plasmid

    #121811

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRAB

    Plasmid

    #121823

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9/KRAB (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAf-CRISPR-phoA

    Plasmid

    #207991

    Purpose

    CRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

    Insert

    phoA
    

    Use

    CRISPR

    Promoter

    Aspergillus flavus U6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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