We narrowed to 21,356 results for: grna

1. LentiGuidPuro-hTP53_sgRNA-1

   Plasmid

   #88853

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYLsgRNA-OsU6b

   Plasmid

   #66196

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   OsU6b

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-cj-E sgRNA

   Plasmid

   #169915

   Purpose

   Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO5.sgRNA.EFS.PAC

   Plasmid

   #57825

   Purpose

   Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   PAC
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6_N7-sgRNA2

   Plasmid

   #190664

   Purpose

   Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   N7 sgRNA2
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pgRNA-CGG

   Plasmid

   #108248

   Purpose

   Lentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   sgRNA targeting GGCGGCGGCGGCGGCGGCGG
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6.3>gRNA.f+e

   Plasmid

   #99139

   Purpose

   Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   gRNA
   

   Use

   CRISPR

   Promoter

   chicken U6.3

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pShHELIX_sgRNA_entry (CJT32)

   Plasmid

   #181782

   Purpose

   Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYLsgRNA-AtU6-29

   Plasmid

   #66203

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYLsgRNA-OsU6a

    Plasmid

    #66194

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    OsU6a

    Available Since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6_B2M_sgRNA(PP7)

    Plasmid

    #232438

    Purpose

    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMGS7 (AAVS1sgRNA)

    Plasmid

    #126582

    Purpose

    A CRISPR/Cas9 construct to target the AAVS1 locus in humans

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    AAVS1 sgRNA
    

    Expression

    Mammalian

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLH-spsgRNA2

    Plasmid

    #64114

    Purpose

    Vector for expression of Sp sgRNA2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp sgRNA2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYLsgRNA-AtU6-1

    Plasmid

    #66202

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. AV15_pCAG.Cas9.gRNA.S1

    Plasmid

    #199259

    Purpose

    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYLsgRNA-AtU3b

    Plasmid

    #66198

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    AtU3b

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. U6.3>Control.gRNA.f+e

    Plasmid

    #99140

    Purpose

    Control gRNA under the regulation of an optimized chicken specific U6 promoter

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    Control gRNA
    

    Use

    CRISPR

    Promoter

    Chicken U6.3

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-Cj-sgRNA

    Plasmid

    #89753

    Purpose

    U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    Cj-SgRNA
    

    Use

    Sgrna expression under u6 promoter

    Promoter

    U6

    Available Since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgRNA 2 GAPDH

    Plasmid

    #83809

    Purpose

    Express Sg-2 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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