We narrowed to 8,669 results for: BLI;

1. pcDNA3.1-hSyncytin-1(C186A)

   Plasmid

   #182450

   Purpose

   hSyncytin-1, fusion deficit and dominant negative mutant (C186A)

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C186A

   Promoter

   CMV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG Retro EGFP Puro

   Plasmid

   #234463

   Purpose

   Control retroviral vector expressing Green Fluorescent Protein. Puromycin selection.

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   Green Fluorescent Protein
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAG Retro EGFP Hygro

   Plasmid

   #234462

   Purpose

   Control retroviral vector expressing Green Fluorescent Protein. Hygromycin selection.

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   Green Fluorescent Protein
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCWU50

   Plasmid

   #232318

   Purpose

   A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch

   Depositor

   Chenggang Wu

   Insert

   Pfdx-E
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   fdx

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

   Plasmid

   #221449

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

   Plasmid

   #221450

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

   Plasmid

   #221451

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-N-TEV-Twin-Strep

   Plasmid

   #222014

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli

   Depositor

   Chiaki Murakami

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site (ENLYFQG) and Twin-strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpec5'

   Plasmid

   #228549

   Purpose

   Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 5' region of aadA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpec3'

    Plasmid

    #228548

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUSE-URA3-Sec7-mEYFP

    Plasmid

    #218968

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mEYFP

    Depositor

    Benjamin Glick

    Insert

    SEC7-mEYFP (SEC7 Budding Yeast)
    

    Use

    Gene replacement

    Tags

    mEYFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUSE-URA-Sec7-mGold

    Plasmid

    #218967

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mGold

    Depositor

    Benjamin Glick

    Insert

    SEC7-mGold (SEC7 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    mGold

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. YIplac211-Kar2-moxGFP2-HDEL

    Plasmid

    #218970

    Purpose

    Gene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2

    Depositor

    Benjamin Glick

    Insert

    KAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    moxGFP2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUSE-URA3-Sec7-msYFP2

    Plasmid

    #218969

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2

    Depositor

    Benjamin Glick

    Insert

    SEC7-msYFP2 (SEC7 Budding Yeast)
    

    Use

    Tags

    msYFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-IRES-tagBFP

    Plasmid

    #219965

    Purpose

    express TagBFP in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    TagBFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiGuideSLC35C1-neo

    Plasmid

    #220868

    Purpose

    encodes CRISPR sgRNA targeting human SLC3C1

    Depositor

    Jennifer Kohler

    Insert

    SLC35C1 (SLC35C1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. icLCT

    Plasmid

    #223179

    Purpose

    Native rat lactase gene (truncated) as an autologous marker (X-Gal, X-Fuc)

    Depositor

    Andreas Stuermer

    Insert

    cytosolic intestinal lactase protein fragment (Lct Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pXR004_puroR

    Plasmid

    #219820

    Purpose

    hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCC1R

    Plasmid

    #213497

    Purpose

    Encodes the receptor array and inducible aiiA gene of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    pcaU
    nahR
    rpaR (rpaR )
    phlF
    cinR
    vanR
    aiiA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid

    #221385

    Purpose

    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

    Depositor

    Junwei Shi

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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