We narrowed to 15,788 results for: LEA

1. FLAG-Pol-II CC

   Plasmid

   #35180

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains C-terminal domain repeats (1-22)X2

   Promoter

   CMV

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pEGFP-C1-PRKAA2ΔC

   Plasmid

   #30307

   Depositor

   Jay Brenman

   Article

   Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

   Insert

   AMP-activated Protein Kinase α2 ΔC (Prkaa1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids Ser 527 to Arg 552

   Promoter

   Available since

   Aug. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pRegev_ExonSkip_LandingPad

   Plasmid

   #204186

   Purpose

   Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   Beta globin splicing minigene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated CAG

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSHS248

   Plasmid

   #87354

   Purpose

   S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/C574S/S867C/N1054C

   Promoter

   T7

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV Cyclin D2-short

   Plasmid

   #21655

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Cyclin D2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV Cyclin D2 long-wt

   Plasmid

   #21646

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Cyclin D2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSET FLIPE-10u

   Plasmid

   #13538

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   A207R in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSSM97: pCMV-HA-NLS-SpuFz1-E498R

   Plasmid

   #205275

   Purpose

   Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498R

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   HA, NLS(Makkerh)

   Expression

   Mammalian

   Mutation

   E498R

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSSM105: pCMV-HA-NLS-SpuFz1-T513K

   Plasmid

   #205276

   Purpose

   Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513K

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   HA, NLS(Makkerh)

   Expression

   Mammalian

   Mutation

   T513K

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSSM58: pCMV-HA-NLS-SpuFz1-D487K

    Plasmid

    #205274

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487K

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    D487K

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSSM59: pCMV-HA-NLS-SpuFz1-D300R

    Plasmid

    #205272

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300R

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    D300R

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. coreR2 (1-387)

    Plasmid

    #115648

    Purpose

    expression of truncated Rag2

    Depositor

    Martin Gellert

    Article

    Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

    Insert

    Rag2 core (1-387) (Rag2 Mouse)
    

    Use

    Tags

    8XHis-MBP-PreScission

    Expression

    Mammalian

    Mutation

    truncated Rag2 (aa 1-387)

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. KBB285

    Plasmid

    #185076

    Purpose

    RFA3-GFP fusion

    Depositor

    Ken Belanger

    Article

    Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

    Insert

    RFA3
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. KBB284

    Plasmid

    #185075

    Purpose

    RFA2-GFP fusion

    Depositor

    Ken Belanger

    Article

    Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

    Insert

    RFA2
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMJ273: pRS426-His-MBP-GtFz1_PSP1

    Plasmid

    #205258

    Purpose

    S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    GtFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMJ127: pRS426-His-MBP-NlovFz2_PSP1

    Plasmid

    #205257

    Purpose

    S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    NlovFz2
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIF251

    Plasmid

    #133935

    Purpose

    Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.

    Depositor

    IIya Finkelstein

    Article

    Kim et al Sci Rep. 2017 May 18;7(1):2071. doi: 10.

    Insert

    Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda
    

    Use

    Λ-phage recombineering plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pKDC.015

    Plasmid

    #184992

    Purpose

    Express -Eco2 RT and ncRNA in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco2: RT and ncRNA(wt), a1/a2 length: 13
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJC175e-DddI

    Plasmid

    #179693

    Purpose

    Mutagenesis plasmid with constitutive bacterial expression of DddI immunity protein

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [SD8] gIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TR-GFP

    Plasmid

    #80591

    Purpose

    Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBA

    Available since

    Sept. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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