We narrowed to 11,272 results for: HAL

1. pSAVED-CHAT-sm_PCaspase

   Plasmid

   #214048

   Purpose

   Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-PCaspase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R456E, D459K, D476K, R479E

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJS-BCD-Strep-SAVED-CHAT

   Plasmid

   #214030

   Purpose

   Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJS-BCD-Strep-dSAVED-CHAT

   Plasmid

   #214031

   Purpose

   Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   H375A, C422A

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJS-BCD-Strep-SAVED-CHAT-sm

   Plasmid

   #214033

   Purpose

   Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   R456E, D459K, D476K, R479E

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJS-BCD-Strep-PCaspase

   Plasmid

   #214034

   Purpose

   Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. fru-T2A-CsChrimson-tdTomato HDR plasmid

   Plasmid

   #141100

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

   Depositor

   Leslie Vosshall

   Article

   Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

   Insert

   fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
   

   Use

   Donor template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP

   Plasmid

   #166020

   Purpose

   mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S )
   

   Use

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30-VRN1-R249E-R289E-R296E

   Plasmid

   #159532

   Purpose

   Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli

   Depositor

   Joshua Mylne

   Article

   King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

   Insert

   VERNALIZATION1 (VRN1 Mustard Weed)
   

   Use

   Tags

   Six-Histidine tag

   Expression

   Bacterial

   Mutation

   Three charge reversal mutations, namely R249E R28…

   Promoter

   T5

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ir7f-T2A-QF2 HDR plasmid

   Plasmid

   #140942

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene

   Depositor

   Leslie Vosshall

   Article

   Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

   Insert

   Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
   

   Use

   Tags

   Expression

   Mutation

   4 point mutations (annotated on plasmid map) were…

   Promoter

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM33_Nsp3e_NAB

    Plasmid

    #156473

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3e_NAB (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCMV_HyPer7RgDAAO

    Plasmid

    #217653

    Purpose

    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    HyPer7 D amino acid oxidase
    

    Use

    Lentiviral

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    Fused DAAO to the C-terminus of HyPer7 using a Gl…

    Promoter

    CMV

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT/TO/HIS/FLAG/HA-HNRNPU

    Plasmid

    #38068

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-Mito-Car-GECO

    Plasmid

    #100765

    Purpose

    Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Lenti-Mito-Car-GECO
    

    Use

    Lentiviral

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-Syn-Jaws-KGC-tdTomato-ER2

    Plasmid

    #153535

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    Syn

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-CaMKII-Jaws-KGC-tdTomato-ER2

    Plasmid

    #153534

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CaMKII

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT/TO/HIS/FLAG/HA-ALKBH5

    Plasmid

    #38073

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo

    Plasmid

    #222636

    Purpose

    Ezrin activation reporter

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    miRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TetO-dCas9-D3A

    Plasmid

    #78254

    Purpose

    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid

    #172314

    Purpose

    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

    Depositor

    Joshua Rosenthal

    Article

    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    

    Use

    Tags

    6xHis, Flag, and Nuclear Localization Signal of S…

    Expression

    Mammalian

    Mutation

    changed the Glutamic acid 488 in the Deaminase Do…

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. TetO-dCas9-mD3A

    Plasmid

    #78255

    Purpose

    Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    E756A

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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