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pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-SpG-P2A-hMLH1dn

Plasmid

#211550

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

Insert

PEmax-SpG-P2A-hMLH1dn

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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PBCAG-EGFR

Plasmid

#40974

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

EGFR (EGFR Human)

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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p5E_unc503

Plasmid

#64020

Purpose

5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

muscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)

Use

Gateway Cloning

Promoter

503-bp promoter of zebrafish unc-45b

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pMT85_P438-mEOS3.2_GentaR

Plasmid

#173894

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

P438

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-HArcLight

Plasmid

#138326

Purpose

chemigenetic voltage indicator

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Insert

HArcLight

Expression

Mammalian

Promoter

CAG

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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Pr-deGFP

Plasmid

#67743

Purpose

Pr promoter expressing deGFP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pYZ960

Plasmid

#187972

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEE391

Plasmid

#149281

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_FT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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PU6::unc-119_sgRNA

Plasmid

#46169

Purpose

unc-119 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

unc-119 targeting sgRNA (unc-119 Synthetic)

Use

CRISPR

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Rheb1 shRNA #2

Plasmid

#26626

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
YFP-NavII-III

Plasmid

#26056

Depositor

Matthew Grubb

Article

Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

Insert

Nav1.2 (Scn2a Rat)

Tags

YFP

Expression

Mammalian

Mutation

The insert codes only for the intracellular loop …

Available Since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pBad33_ISYmu1_reRNA

Plasmid

#205358

Purpose

Bacterial expression of ISYmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISYmu1 TnpB

Expression

Bacterial

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TRPML1-MYC

Plasmid

#18824

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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Flag Rab12 Q101L

Plasmid

#86788

Purpose

mammalian expression of Rab12 Q101L, constitutively GTP-bound mutant

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Tags

Flag

Expression

Mammalian

Mutation

Q101L, constitutively GTP-bound mutant

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEAK10-His-Myc-Kal12

Plasmid

#25442

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal12

Tags

His and Myc

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

Plasmid

#179008

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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punc503GFP

Plasmid

#64021

Purpose

503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expression

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

unc-45b: GFP (unc45b Zebrafish)

Use

Gateway Cloning

Promoter

503-bp promoter of zebrafish unc-45b

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Km-SceI-pheS

Plasmid

#111610

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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