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Showing: 3501 - 3520 of 19663 results

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pET-3c-rhVTN_FL

Plasmid

#30223

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin without deletions (VTN Human)

Expression

Bacterial

Mutation

No deletions

Available Since

May 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2PHR

Plasmid

#28244

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

cry2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Available Since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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Burden Monitor lambda version

Plasmid

#66073

Purpose

The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

GFP

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
MMW948: CAG-DmrC-NLS-3xFLAG-VPR

Plasmid

#104565

Purpose

Mammalian expression vector for DmrC fused to VPR activator

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

DmrC-VPR

Tags

3x FLAG and NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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modRNAc0-Cas9-2A-Puro

Plasmid

#172855

Purpose

IVT template plasmid for making modified Cas9Puro RNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

Cas9-2A-Puro

Use

Ivt template plasmid

Tags

2A-Puro

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-CERP2-MG-IRES2-mNeonGreen

Plasmid

#175508

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCIB1(deltaNLS)-pmGFP

Plasmid

#28240

Purpose

Expresses CIB1 fused to plasma membrane-localized EGFP

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-CIBN-BLITz1-TetR-VP16

Plasmid

#89878

Purpose

expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

TM-CIBN-TevN-BLITz1-TetR-VP16

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

Plasmid

#87694

Purpose

Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

ERT2-PhoCl-Cre-PhoCl-ERT2

Expression

Mammalian

Promoter

Chicken beta actin

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

Plasmid

#85069

Purpose

Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH-PLCD1 (PLCD1 Human)

Tags

IRES-sYFP2-PH and mScarlet

Expression

Mammalian

Mutation

PLCD aa 1–170

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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cmv-sfGFP-GB1-scAB-ubc-nls-pcp-10xSunTag(SRv1.1-2P)

Plasmid

#185801

Purpose

one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-sfGFP-GB1-scAB--ubc-nls-pcp-10xSunTag

Expression

Mammalian

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-NES-CRY2PHR-TevC

Plasmid

#89877

Purpose

expresses NES-CRY2PHR-TevC of BLITz system

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

NES-CRY2PHR-TevC

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-218-2-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85881

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-218-2-3p Sensor (MIR218-2 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

Plasmid

#84247

Purpose

Expresses direct fusion VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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p4B5-EGFP

Plasmid

#140324

Purpose

Detection of Dengue virus infection in live cells

Depositor

Alan Rothman

Article

Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

Insert

DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP

Tags

eGFP

Expression

Mammalian

Mutation

2 conservative n.t. differences near 5' end …

Promoter

CMV

Available Since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-vioABECD

Plasmid

#66535

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Consensus T7

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-bArrestin2-TevC-P2A-TdTomato

Plasmid

#89869

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

Synthetic Biology

Expression

Mammalian

Mutation

R173H in TdTomato (please see depositor comments …

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5L18-CtermQ3

Plasmid

#51440

Purpose

Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) Q3 C-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K788Q, R792Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5R18-CtermQ3

Plasmid

#51441

Purpose

Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) Q3 C-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K788Q, R792Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pBluescriptII[attB/Ins1]

Plasmid

#74100

Purpose

"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Phi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators

Expression

Bacterial

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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