We narrowed to 10,167 results for: otos

1. pSpCas9 (PX165)

   Plasmid

   #48137

   Purpose

   Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. TLCV2-sgHPDL #3

   Plasmid

   #174165

   Purpose

   knock out HPDL in human cell lines

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   sgRNA against HPDL
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TLCV2-sgHPDL #9

   Plasmid

   #174166

   Purpose

   knock out HPDL in human cell lines

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   sgRNA against HPDL
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Gdf6

   Plasmid

   #163598

   Purpose

   Expresses GDF-6 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Gdf6 (Gdf6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY2F

   Plasmid

   #53285

   Purpose

   Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   lcyE (LUT2 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEG BacMam C term 3C eGFP StrepII

   Plasmid

   #160686

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   3C eGFP StrepII

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-TRE-Tight2-AkaLuc

   Plasmid

   #186186

   Purpose

   AAV vector packaging AkaLuciferase under the TRE promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

   Insert

   AkaLuc luciferase
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE-Tight2

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209666

   Purpose

   Non-responsive control

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. NLS-SuperNova

   Plasmid

   #131858

   Purpose

   Expresses SuperNova fused to nuclear localization signal (NLS)

   Depositor

   Geert van den Bogaart

   Article

   Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

   Insert

   SuperNova
   

   Use

   Tags

   Myc tag and c-myc nuclear localization signal (NL…

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEG BacMam N term StrepII eGFP 3C

    Plasmid

    #160683

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    StrepII eGFP 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSC-GFP-NES-EGFP

    Plasmid

    #221810

    Purpose

    Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    GFP-NES-EGFP
    

    Use

    Lentiviral

    Tags

    NES (internal tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209724

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-H2B-GFP

    Plasmid

    #116869

    Purpose

    Nuclear localized GFP

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEN276

    Plasmid

    #69150

    Purpose

    Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

    Depositor

    Pierre Germon

    Article

    Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    frr

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Akaluc-N1

    Plasmid

    #120371

    Purpose

    Akaluc luciferase mammalian expression in -N1 backbone

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Akaluc (human optimized)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human codon optimized

    Promoter

    CMV

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-tdTomato-P2A-paCaMKII

    Plasmid

    #165431

    Purpose

    Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    tdTomato-P2A-paCaMKII
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cag.Luc.puro

    Plasmid

    #74409

    Purpose

    Constitutive Luciferase expression in mammalian cells along with puromycin selection

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al EMBO Mol Med. 2017 May;9(5):672-686. doi

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-R-GECO1.2

    Plasmid

    #45494

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET17_K560_GFP_His

    Plasmid

    #15219

    Depositor

    Ron Vale

    Article

    Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

    Insert

    Human neuronal kinesin heavy chain (KIF5B Human)
    

    Use

    Tags

    GFP and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEG BacMam C term 3C eGFP His8

    Plasmid

    #160687

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    3C eGFP His8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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