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mEos2-Talin-H-18

Plasmid

#57422

Purpose

Localization: Talin Head Domain, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin H

Tags

mEos2

Expression

Mammalian

Mutation

Talin Head Domain

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-VASP-5

Plasmid

#57433

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

VASP

Tags

mEos2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-Paxillin-22

Plasmid

#57653

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
U6>Ebf.774(F+E)

Plasmid

#59990

Purpose

U6 promoter driving Ebf.774 (F+E) sgRNA

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

Ebf.774 (F+E) sgRNA

Use

CRISPR

Promoter

Ciinte.U6

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50010

Plasmid

#50310

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal Myc tag (4x Myc)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH51266

Plasmid

#50267

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Claspin-FL-wt

Plasmid

#31621

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

Flag, GST, and His

Expression

Bacterial

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfPar3_3

Plasmid

#26705

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Par3 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Par3 RNAi

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfTuba_1

Plasmid

#26717

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Tuba RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-Blast-cfCdc42_2

Plasmid

#26703

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Cdc42

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to canine Cdc42

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pCS2-Opto-Alk8

Plasmid

#207615

Purpose

Zebrafish Alk8 BMP receptor kinase domain fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

Alk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Vaucheria frigida, Zebrafish)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSB1C3-pT3-tetR-sfGFP

Plasmid

#140871

Purpose

T3 promoter driven synthesis of TetR-sfGFP

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-sfGFP expressed with T3 promoter

Use

Synthetic Biology

Tags

sfGFP

Expression

Bacterial

Promoter

T3

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-mTurquoise2-WPRE

Plasmid

#104103

Purpose

Expresses mTurquoise2 under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

mTurquoise2

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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f1H (264)

Plasmid

#24425

Depositor

Steven Rosen

Article

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF1 (SULF1 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

22aa N-terminal truncation

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
mg180

Plasmid

#34660

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B0;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pET28Teb1

Plasmid

#28173

Depositor

Kathleen Collins

Article

Min et al Mol Cell. 2009 Nov 25. 36(4):609-19.

Insert

Teb1

Tags

6xhistidine

Expression

Bacterial

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Pthl_fU

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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H2B-LSSmGFP

Plasmid

#186539

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Human Histone 2B (D26G and V119I mutations)

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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VGluT1-SNAPtag

Plasmid

#107371

Purpose

Synaptic vesicle protein VGluT1 fused to the SNAPtag domain

Depositor

David Perrais

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

Vesicular Glutamate transporter 1 (Slc17a7 Rat)

Tags

SNAPtag, 104 aa before C term

Expression

Mammalian

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1-IRES2-EGFP

Plasmid

#170820

Purpose

human GABAA receptors alpha1 subunit and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit (GABRA1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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