-
Plasmid#175224PurposeXenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOL092W (CEN.PK strain)
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Cdc42/13C-FKBP-MBP
Plasmid#214291PurposeExpress split-Cdc42 fragment fused with FKBP and MBPDepositorInsertSplit-Cdc42 fragment fused with FKBP (CDC42 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationCdc42-Q61LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-FRB-RhoA/N12
Plasmid#214298PurposeExpress split-RhoA fragment fused with FRB and MBPDepositorInsertSplit-RhoA fragment fused with FRB (RHOA Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RhoA/13C-FKBP-MBP
Plasmid#214299PurposeExpress split-RhoA fragment fused with FKBP and MBPDepositorInsertSplit-RhoA fragment fused with FKBP (RHOA Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRhoA-Q63LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-FRB-Cdc42/N12
Plasmid#214290PurposeExpress split-Cdc42 fragment fused with FRB and MBPDepositorInsertSplit-Cdc42 fragment fused with FRB (CDC42 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 DomI + DomII pcDNA3
Plasmid#206094Purposeexpression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channelDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Bn CAAX pcDNA3
Plasmid#206088PurposeCav2.2 N-terminus (amino acids 1-95) with a C-terminal tag of the final 10 aa of H.Ras containing CAAX motifDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 BBS pcDNA3
Plasmid#206106Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single Bungarotoxin Binding Site motif inserted after aa549HADepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA W391A pcDNA3
Plasmid#206071Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENeCFPCNAL2 pc922
Plasmid#167563PurposeFor construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-IGFBP2-Clover
Plasmid#191007PurposeGateway entry vector with IGFBP2 tagged with CloverDepositorInsertInsulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
UseGateway entry vectorTagsCloverExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-PolB(T304I)-Puro
Plasmid#177143PurposeLentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S159A
Plasmid#131520PurposeExpresses MKP-3 with S159A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Serine 159 to AlaninePromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_m-AKAR5
Plasmid#114139PurposeMammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 C-terminally tagged by KRas C tail
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cyt-AKAR5
Plasmid#114138PurposeMammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 with C-terminal nuclear export signal (NES)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR2Q191y
Plasmid#101029Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR2Q207y
Plasmid#101024Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromotercMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR2Q133c
Plasmid#101025Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromoterCMVAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL2
Plasmid#67459PurposeMammalian expression of hArl2 with stop codon (native expression)DepositorInsertARL2 (ARL2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL4
Plasmid#67461PurposeMammalian expression of hArl4 with stop codon (native expression)DepositorInsertARL4 (ARL4A Human)
UseTagsExpressionMammalianMutationbp 105 A to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL5A
Plasmid#67462PurposeMammalian expression of hArl5 with stop codon (native expression)DepositorInsertARL5A (ARL5A Human)
UseTagsExpressionMammalianMutationbp 70 C to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL6
Plasmid#67464PurposeMammalian expression of hArl6 with stop codon (native expression)DepositorInsertARL6 (ARL6 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL14
Plasmid#67469PurposeMammalian expression of hArl14 with stop codon (native expression)DepositorInsertARL14 (ARL14 Human)
UseTagsExpressionMammalianMutationbp 172 C to T; aa L58FPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL11
Plasmid#67470PurposeMammalian expression of hArl11 with stop codon (native expression)DepositorInsertARL11 (ARL11 Human)
UseTagsExpressionMammalianMutationbp 261 G to A; silent mutation; bp 442 T to C; aa…PromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL4-V5-His6
Plasmid#67440PurposeMammalian expression of hArl4 with C-term V5 and His6 tagDepositorInsertARL4 (ARL4A Human)
UseTagsHis and V5ExpressionMammalianMutationbp 105 A to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL5A-V5-His6
Plasmid#67441PurposeMammalian expression of hArl5 with C-term V5 and His6 tagDepositorInsertARL5A (ARL5A Human)
UseTagsHis and V5ExpressionMammalianMutationbp 70 C to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL5B-V5-His6
Plasmid#67442PurposeMammalian expression of hArl5b with C-term V5 and His6 tagDepositorInsertARL5B (ARL5B Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL4C-V5-His6
Plasmid#67444PurposeMammalian expression of hArl4c with C-term V5 and His6 tagDepositorInsertARL7 (ARL4C Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL14-V5-His6
Plasmid#67448PurposeMammalian expression of hArl14 with C-term V5 and His6 tagDepositorInsertARL14 (ARL14 Human)
UseTagsHis and V5ExpressionMammalianMutationbp 172 C to T; aa L58FPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL11-V5-His6
Plasmid#67449PurposeMammalian expression of hArl11 with C-term V5 and His6 tagDepositorInsertARL11 (ARL11 Human)
UseTagsHis and V5ExpressionMammalianMutationbp 442 T to C; aa C148RPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARFRP1-V5-His6
Plasmid#67450PurposeMammalian expression of hArfrp with C-term V5 and His6 tagDepositorInsertARFRP1 (ARFRP1 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF3
Plasmid#67454PurposeMammalian expression of hArf3 with stop codon (native expression)DepositorInsertARF3 (ARF3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF5
Plasmid#67456PurposeMammalian expression of hArf5 with stop codon (native expression)DepositorInsertARF5 (ARF5 Human)
UseTagsExpressionMammalianMutationbp 477 T to C; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF3-V5-His6
Plasmid#67412PurposeExpression of hArf3 with C-term V5 and His6 tag in E. coliDepositorInsertARF3 (ARF3 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF4-V5-His6
Plasmid#67413PurposeExpression of hArf4 with C-term V5 and His6 tag in E. coliDepositorInsertARF4 (ARF4 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL1-V5-His6
Plasmid#67416PurposeExpression of hArl1 with C-term V5 and His6 tag in E. coliDepositorInsertARL1 (ARL1 Human)
UseTagsHis and V5ExpressionBacterialMutationbp 231 C to T; silent mutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL2-V5-His6
Plasmid#67417PurposeExpression of hArl2 with C-term V5 and His6 tag in E. coliDepositorInsertARL2 (ARL2 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL5A-V5-His6
Plasmid#67420PurposeExpression of hArl5 with C-term V5 and His6 tag in E. coliDepositorInsertARL5A (ARL5A Human)
UseTagsHis and V5ExpressionBacterialMutationbp 70 C to T; silent mutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL8A-V5-His6
Plasmid#67424PurposeExpression of hArl8 with C-term V5 and His6 tag in E. coliDepositorInsertARL8A (ARL8A Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL14-V5-His6
Plasmid#67427PurposeExpression of hArl14 with C-term V5 and His6 tag in E. coliDepositorInsertARL14 (ARL14 Human)
UseTagsHis and V5ExpressionBacterialMutationbp 172 C to T; aa L58FPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only