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STAT3(Y705F)-TAL-Luc

Plasmid

#46933

Purpose

Luciferase Reporter for mutant STAT3

Depositor

Afshin Dowlati

Article

Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60.

Insert

STAT3(Y705F) (STAT3 Human)

Use

Luciferase

Tags

FLAG

Mutation

Tyrosine 705 to Phenylalanine

Promoter

TA Luciferase

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only
3544 pMIG Bcl-2

Plasmid

#8793

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-2 (BCL2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK3b(Glu)

Plasmid

#50449

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

CMV

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 NPY pHLeon

Plasmid

#235176

Purpose

Plasmid expressing NPY tag with pH sensor, pHLeon (pH-Lemon with mNeonGreen), in mammalian cells

Depositor

Kate White

Article

Lin et al ACS Sens. 2024 Nov 22;9(11):6032-6039. d

Insert

NPY-pHLeon (NPY Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET21 gamma-tubulin

Plasmid

#101825

Purpose

production of gamma-tubulin in bacteria

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

His

Expression

Bacterial

Mutation

Whole protein

Promoter

T7

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-T-GECO1

Plasmid

#191616

Purpose

Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

Depositor

Robert Campbell

Article

Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

Insert

T-GECO1

Tags

His tag

Expression

Bacterial

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9

Plasmid

#133909

Purpose

Bacterial expression of His6-Ubc9

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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nLuc-PTHR1

Plasmid

#162577

Purpose

Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

Depositor

Sam Gellman

Article

Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

Insert

Parathyroid Hormone Receptor 1 (PTH1R Human)

Tags

Nanoluc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5b-Myc

Plasmid

#186614

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5b (KIF5B Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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CFP-CD82

Plasmid

#1818

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Tags

CFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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FL-fibulin-3-V5-lentiviral-GFP

Plasmid

#29703

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only
Keasling-2337

Plasmid

#100168

Purpose

Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

Depositor

Jay Keasling

Article

Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

Insert

lacIq-Ptrc-ADS

Expression

Bacterial

Promoter

trc

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCF827-recipient_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211692

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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YFP-CD82

Plasmid

#1819

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Tags

YFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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Stat3 S727A pRc/CMV

Plasmid

#8708

Depositor

Jim Darnell

Article

Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

Insert

Stat3 S727A (Stat3 Mouse)

Expression

Mammalian

Mutation

S727A (exhibits o serine phosphorylation either c…

Available Since

Aug. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Expression

Mammalian

Promoter

Tet responsive element

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5L18-CtermQ3

Plasmid

#51440

Purpose

Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) Q3 C-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K788Q, R792Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5R18-CtermQ3

Plasmid

#51441

Purpose

Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) Q3 C-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K788Q, R792Q and R801Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pEYFP-hDF

Plasmid

#72584

Purpose

human dysferlin full length tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5a-Myc

Plasmid

#186613

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform A fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5A (KIF5A Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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